{"product_id":"abcam-ab315050","title":"Abcam, ab315050, pan-HMR VEGFC ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 10 x 96Tests\u003cbr\u003e\npan-HMR VEGFC ELISA Kit is a single-wash 90-min Simplestep used to quantify pan-HMR VEGFC with a sensitivity of 4.375 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Serum, Heparin Plasma, EDTA Plasma, Citrate plasma, Cell culture supernatant,\u003cbr\u003e\nReacts with:Rat, Human, Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 4.375 pg\/mL,\u003cbr\u003e\nRange:5.859 - 375 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\npan-HMR VEGFC ELISA Kit ab315050 is a rapid single-wash 90-min Sandwich ELISA to measure pan-HMR VEGFC in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 4.375 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\npan-HMR VEGFC SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\npan-HMR VEGFC ELISA Kit ab315050 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nVEGFC also known as Vascular Endothelial Growth Factor C is a member of the VEGF family involved in angiogenesis and lymphangiogenesis. It has a molecular mass of approximately 35 kDa. VEGFC is expressed in various tissues with high levels in the heart lung and muscle. It acts primarily through its interaction with the VEGFR-3 receptor stimulating the formation of blood and lymphatic vessels. This protein plays an important role in the development and maintenance of the circulatory and lymphatic systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nVEGFC contributes to the regulation of vascular and lymphatic endothelial cell functions. It is not known to be part of a larger protein complex but exerts its functions through interactions with receptors such as VEGFR-3. By promoting cell proliferation and migration VEGFC assists in the repair and regeneration of tissues. Its role in fluid balance is essential as it helps maintain tissue equilibrium and immune function.\u003cbr\u003e\nPathways\u003cbr\u003e\nVEGFC is integral to the VEGF signaling and lymphatic vessel development pathways. Within these processes VEGFC works alongside other proteins such as VEGFA and VEGFB to modulate vascular permeability and vessel formation. The interaction between VEGFC and the VEGFR-3 receptor specifically mediates lymphatic endothelial cell activation and function linking it to vital physiological processes like inflammation and tissue repair.\u003cbr\u003e\nVEGFC is associated with cancer and lymphedema. Its role in promoting angiogenesis is critical in tumor growth and metastasis with its overexpression often linked to cancer progression. Furthermore insufficient VEGFC activity contributes to the development of lymphedema due to inadequate lymphatic drainage. In cancer pathology VEGFC works together with VEGFR-3 contributing to the abnormal growth of lymphatic vessels which in turn facilitates cancer cell dissemination.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843626160297,"sku":"ab315050","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab315050","provider":"Iright","version":"1.0","type":"link"}