{"product_id":"abcam-ab315067","title":"Abcam, ab315067, pan-HMR GSK3 beta ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\npan-HMR GSK3 beta ELISA Kit is a single-wash 90-min Simplestep used to quantify pan-HMR GSK3 beta with a sensitivity of 34.77 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human, Mouse, Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 34.77 pg\/mL,\u003cbr\u003e\nRange:312.5 - 20000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\npan-HMR GSK3 beta SimpleStep ELISA\u003cbr\u003e\nkit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GSK3 beta protein in Cell Lysate. Quantitate pan-HMR GSK3 beta with 34.77 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA\u003cbr\u003e\ntechnology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details. Our SimpleStep ELISA\u003cbr\u003e\ntechnology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA\u003cbr\u003e\nmicroplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlycogen synthase kinase 3 beta (GSK3B) also referred to as GSK-3 beta is a serine\/threonine kinase with an approximate mass of 46 kDa. It is widely expressed in various tissues including the brain heart and liver highlighting its significance in diverse cellular processes. As a protein kinase GSK3B phosphorylates serine and threonine residues on its substrate proteins controlling their activity and stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGSK3B participates in numerous cellular functions such as glycogen metabolism neuronal signaling and cellular adhesion. It forms part of a complex that is important for the regulation of various transcription factors and enzymes. GSK3B interacts with several cellular proteins to modulate their activity such as influencing the beta-catenin pathway that impacts cell growth and division. This regulation is essential for maintaining proper cellular function and response to external stimuli.\u003cbr\u003e\nPathways\u003cbr\u003e\nGSK3B plays a significant role in the Wnt signaling pathway and the insulin signaling pathway. These pathways are essential for cellular development differentiation and energy metabolism. GSK3B interacts closely with proteins like beta-catenin in the Wnt pathway and regulates glycogen synthase in the insulin signaling pathway. Through these interactions it ensures precise control over cellular proliferation survival and glucose metabolism.\u003cbr\u003e\nGSK3B has significant connections to disorders like Alzheimer's disease and type 2 diabetes. In Alzheimer's disease abnormal activity of GSK3B is linked to the hyperphosphorylation of tau protein which contributes to neurofibrillary tangles. In type 2 diabetes GSK3B's involvement in the insulin signaling pathway can impact insulin resistance. Its dysregulation also affects proteins like the tau protein in neurodegenerative disorders and insulin receptor substrate proteins in metabolic disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843625767081,"sku":"ab315067","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab315067","provider":"Iright","version":"1.0","type":"link"}