{"product_id":"abcam-ab315974","title":"Abcam, ab315974, Anti-EVI1 antibody [EPR26816-18] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal EVI1 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR26816-18,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab315974 is the carrier-free version of\u003cbr\u003e\nab315973\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEVI1 also known as MECOM or MECOM way is a transcription factor with a molecular mass of approximately 145 kDa. It is primarily expressed in hematopoietic stem cells and various tissues including lung and kidney. EVI1 plays a role in regulating gene expression by binding to specific DNA sequences influencing the transcription of target genes. Its expression is tightly controlled during development and aberrant expression frequently correlates with pathological conditions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEVI1 participates in the regulation of cell growth and differentiation. As part of a complex with other proteins EVI1 modulates transcriptional outcomes necessary for maintaining stem cell identity and preventing premature differentiation. It helps balance cell cycle progression and apoptosis impacting cellular responses to external stimuli. EVI1 cooperates with other transcription factors in gene regulation networks that control developmental processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nEVI1 interacts with several signaling pathways notably the TGF-beta and MAPK pathways. It influences TGF-beta signaling by modulating transcription factors such as SMADs which are central to this pathway. EVI1's association with MAPK signaling impacts cell proliferation and differentiation integrating multiple signaling inputs to coordinate cellular outcomes. Through these pathways EVI1 interacts with proteins like SMAD3 and ERK essential players in its regulatory circuit.\u003cbr\u003e\nEVI1 shows a strong connection with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). In AML aberrant expression or rearrangements involving EVI1 result in blockages of normal myeloid differentiation leading to leukemic transformation. EVI1 also associates with transcription factors like GATA2 in these diseases intensifying the disruption of normal hematopoietic functions. Understanding EVI1's role offers insights into the molecular mechanisms underlying these malignancies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46856267366569,"sku":"ab315974","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab315974","provider":"Iright","version":"1.0","type":"link"}