{"product_id":"abcam-ab316286","title":"Abcam, ab316286, Anti-HCoV-229E Nucleocapsid protein antibody [abd210] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nHuman Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for ICC\/IF, I-ELISA and reacts with Transfected cell line - Human coronavirus 229E, Recombinant fragment - Human coronavirus 229E samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Human,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:abd210,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human coronavirus 229E,\u003cbr\u003e\nApplications:I-ELISA, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody does not cross-react with recombinant Human coronavirus NL63 nucleocapsid, MERS-CoV nucleocapsid, SARS-CoV nucleocapsid, Human coronavirus OC43 nucleocapsid and Human coronavirus HKU1-Cov nucleocapsid.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab316286 is the carrirer-free version of\u003cbr\u003e\nab316285\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe HCoV-229E Nucleocapsid protein also known as the N protein plays a mechanical role within the human coronavirus 229E (HCoV-229E). This protein has a mass of around 45 kDa and is expressed in the cytoplasm of infected host cells. Its primary function lies in binding to the viral RNA genome forming a ribonucleoprotein complex that protects the RNA and contributes to the stability of the viral genome. The HCoV-229E nucleocapsid protein is important for the correct assembly and packaging of the viral RNA into new virions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe N protein directly impacts viral replication and transcription. It forms part of a larger ribonucleoprotein complex that organizes the viral genome into a functional and protected structure. This complex interacts with other viral proteins to facilitate the replication process. The N protein also boosts the efficiency of the viral RdRp (RNA-dependent RNA polymerase) in transcribing the RNA genome ensuring that viral replication proceeds efficiently and effectively within host cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe N protein integrates into the coronavirus replication-transcription complex. It coordinates with the viral RdRp and other non-structural proteins of the virus to ensure a streamlined replication process. This protein interacts with cellular pathways that include stress response and immune pathways potentially modulating host immune responses to benefit viral survival and replication. These interactions with host cell pathways may influence the efficiency of viral replication and the pathogenicity of the virus.\u003cbr\u003e\nHCoV-229E Nucleocapsid protein connects to common cold manifestations and in some cases exacerbates chronic respiratory disorders. The N protein’s interactions with other viral proteins like the Spike protein might enhance viral entry and infectivity which could contribute to varying severity of infection. Understanding the role of the N protein in these contexts is important for developing therapeutic strategies and improving disease management for infections caused by HCoV-229E.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854641483945,"sku":"ab316286","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab316286","provider":"Iright","version":"1.0","type":"link"}