{"product_id":"abcam-ab3613","title":"Abcam, ab3613, Anti-HEC1\/HEC antibody [9G3]","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nAnti-HEC1\/HEC antibody [9G3] (ab3613) is a mouse monoclonal antibody detecting HEC1\/HEC in  Western Blot, Flow Cytometry, IP, ICC\/IF . Suitable for  Human, Pig . - Over 110 publications - Trusted since 2003\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:9G3,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nLight chain type:unknown,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human, Pig,\u003cbr\u003e\nApplications:ICC\/IF, WB, IP, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human NDC80 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O14777\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-HEC1\/HEC antibody [9G3] (ab3613) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Pig samples.\u003cbr\u003e\nWhat is the molecular weight of HEC1\/HEC?\u003cbr\u003e\nAnti-HEC1\/HEC [9G3] (ab3613) specifically detects a band for HEC1\/HEC (UniProt: O14777) at a molecular weight of 74kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-HEC1\/HEC [9G3] (ab3613) was first used in a scientific publication in 2003 and has been cited over 110 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-HEC1\/HEC [9G3] (ab3613) has over 10 independent reviews from customers.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from TCS, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHEC1 also known as NDC80 or HEC is an important component of the kinetochore a protein complex importantly involved in chromosome segregation during cell division. It is often referred to as HEC-1 with a molecular mass of approximately 80 kDa. HEC1 is expressed ubiquitously in proliferating cells and it plays an important role in the accurate alignment and segregation of chromosomes during mitosis. The protein is found at centromeres where it is essential for proper attachment of microtubules.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIn the context of cell division HEC1 functions as part of the NDC80 complex a critical ensemble of four proteins that include NUF2 SPC24 and SPC25. This complex is vital for microtubule binding and kinetochore function. HEC1 as a subunit of NDC80 ensures kinetic stability and maintains the structural integrity of the kinetochore during the cell cycle. Its function is necessary for accurate chromosome alignment on the metaphase plate which prevents missegregation and aneuploidy.\u003cbr\u003e\nPathways\u003cbr\u003e\nHEC1 is actively involved in the spindle assembly checkpoint (SAC) a safety mechanism that prevents anaphase onset until all chromosomes are properly aligned. This pathway is important in regulating cell cycle progression and preventing genomic instability. HEC1 also interacts with proteins like BUB1 and MAD2 which are integral to the SAC pathway. HEC1’s associations highlight its role in maintaining mitotic accuracy and integrity in dividing cells.\u003cbr\u003e\nHEC1 has a significant connection to cancer. Aberrant expression or malfunction of HEC1 contributes to chromosomal instability a hallmark of cancer progression. Its interactions with oncogenes and tumor suppressor proteins like TP53 can exacerbate this instability leading to unregulated cell division and tumor growth. Additionally HEC1's role in the cell cycle and proliferation links it to disorders like aneuploidies where chromosome number errors occur further implicating its importance in maintaining chromosomal balance.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855707820201,"sku":"ab3613","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab3613","provider":"Iright","version":"1.0","type":"link"}