{"product_id":"abcam-ab39401","title":"Abcam, ab39401, Caspase-3 Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nCaspase-3 Assay Kit (Colorimetric) is a rapid and easy to use kit to assay the activity of caspases that recognisze the DEVD sequence. - Colorimetric assay - 400 nm readout - works on standard microplate readers - Determine the fold-increase in Caspase-3 activity in just over 2 hours - Cited in over 320 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Lysate, Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nAssay time:2h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCaspase-3 Assay Kit (Colorimetric) ab39401 allows the rapid, sensitive and accurate measurement of Caspase-3 activity in cell lysates.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThe Caspase-3 assay protocol is based on the formation of the chromophore p-nitroaniline (p-NA) by cleavage from the labeled substrate DEVD-pNA. The p-NA can be quantified using a spectrophotometer or a microtiter plate reader reading absorbance at 400 or 405 nm.\u003cbr\u003e\nComparison of the absorbance of p-NA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3 activity.\u003cbr\u003e\nCaspase-3 Assay protocol summary\u003cbr\u003e\n- Add samples to wells\u003cbr\u003e\n- Add reaction buffer and DEVD-p-NA substrate and incubate for 60-120 min at 37°C\u003cbr\u003e\n- Analyze with microplate reader\u003cbr\u003e\nNote: Due to the nature of the substrate, this assay also detects caspase-7 activity.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843573534889,"sku":"ab39401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab39401","provider":"Iright","version":"1.0","type":"link"}