{"product_id":"abcam-ab416","title":"Abcam, ab416, Anti-EAAT1 antibody","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-EAAT1 antibody (ab416) is a rabbit polyclonal antibody detecting EAAT1 in  Western Blot, IHC-P . Suitable for  Human, Rat . - Over 140 publications - Trusted since 1999\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Rat, Human,\u003cbr\u003e\nApplications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Rat Slc1a3. The exact immunogen used to generate this antibody is proprietary information.P24942\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-EAAT1 antibody (ab416) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB in human, rat samples.\u003cbr\u003e\nAnti-EAAT1 antibody (ab416) specifically detects EAAT1 (UniProt ID: P24942; Molecular weight: 60kDa) and is sold in 100 µg selling sizes.\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality validation processes ensure Anti-EAAT1 antibody (ab416) has high sensitivity and specificity.\u003cbr\u003e\nAnti-EAAT1 antibody (ab416) has been cited over 148 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAnti-EAAT1 antibody (ab416) has 13 independent reviews from customers.\u003cbr\u003e\nGlutamate-aspartate transporter is also known as GLAST.\u003cbr\u003e\nWe are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1. Lot numbers other than GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEAAT1 also known as Excitatory Amino Acid Transporter 1 or anti-GLAST plays a critical role in mediating the uptake of glutamate from the synaptic cleft into glial cells. This transporter helps maintain the delicate balance of excitatory neurotransmission in the central nervous system. EAAT1 has a molecular mass of approximately 57 kDa. You will find EAAT1 expressed predominantly in astrocytes within the brain and retina where it facilitates the regulation of extracellular concentrations of glutamate preventing excitotoxicity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEAAT1 is instrumental in maintaining low levels of extracellular glutamate acting as a protective mechanism against neurotoxicity. This transporter does not operate as part of a larger protein complex instead functioning independently to efficiently clear synaptic glutamate. By ensuring rapid uptake of glutamate EAAT1 helps maintain normal synaptic transmission and neuronal communication. The action of EAAT1 is important for preventing excessive activation of glutamate receptors which can lead to cellular damage.\u003cbr\u003e\nPathways\u003cbr\u003e\nEAAT1 is heavily involved in the glutamatergic neurotransmission pathway which is essential for various brain functions including learning and memory. EAAT1 acts in coordination with other proteins like EAAT2 to manage glutamate levels within the synaptic cleft. Another pathway where EAAT1 plays a role is the glutamate-glutamine cycle where it works alongside proteins such as glutamine synthetase to recycle glutamate and sustain neurotransmitter balance. This interplay supports neuronal health and protects against glutamate excitotoxicity.\u003cbr\u003e\nEAAT1 has been implicated in neurological conditions such as epilepsy and Alzheimer's disease. The dysfunction or altered expression of EAAT1 can lead to insufficient glutamate clearance contributing to the pathophysiology of these disorders. In epilepsy the decreased function of EAAT1 may result in abnormally high levels of synaptic glutamate enhancing the risk of seizures. Similarly in Alzheimer's altered EAAT1 activity can exacerbate neurodegenerative processes. EAAT1's role is tightly linked with proteins like NMDA receptors and EAAT2 which further influence disease progression by affecting glutamate dynamics.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855721582761,"sku":"ab416","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab416","provider":"Iright","version":"1.0","type":"link"}