{"product_id":"abcam-ab46028","title":"Abcam, ab46028, Human IL-1 alpha ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 2 x 96Tests\u003cbr\u003e\nHuman IL-1 alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-1 alpha in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 10 pg\/mL,\u003cbr\u003e\nRange:31.25 - 1000 pg\/mL,\u003cbr\u003e\nAssay time:3h 45m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's IL-1 alpha Human\u003cbr\u003e\nin vitro\u003cbr\u003e\nELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 alpha in cell culture supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognize both natural and recombinant Human IL-1a.\u003cbr\u003e\nA monoclonal antibody specific for IL-1 alpha has been coated to the wells of the microtiter strip plate provided. Samples, including standards of known IL-1 alpha concentrations and unknown are pipetted into these wells. During incubation, binding of IL-1 alpha to the capture antibodies is completed and then any excess unbound analyte is removed by washing. A biotinylated monoclonal antibody specific for IL-1 alpha is then incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the sample.\u003cbr\u003e\nThis kit will recognize both endogenous and recombinant Human IL-1 alpha.\u003cbr\u003e\nGet higher sensitivity in only 90 minutes with Human IL-1 alpha ELISA Kit (\u003cbr\u003e\nab178008\u003cbr\u003e\n) from our SimpleStep ELISA\u003cbr\u003e\nrange.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855677542569,"sku":"ab46028","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab46028","provider":"Iright","version":"1.0","type":"link"}