{"product_id":"abcam-ab46029","title":"Abcam, ab46029, Human IL-6 Receptor ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 2 x 96Tests\u003cbr\u003e\nHuman IL-6 Receptor ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-6 Receptor in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 5 pg\/mL,\u003cbr\u003e\nRange:31.25 - 1000 pg\/mL,\u003cbr\u003e\nAssay time:1h 45m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's IL-6 Receptor (Interleukin-6 Receptor) Human\u003cbr\u003e\nin vitro\u003cbr\u003e\nELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-6 Receptor in Human serum, plasma, buffered solutions and cell culture medium.\u003cbr\u003e\nA monoclonal antibody specific for IL-6 Receptor has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 Receptor concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 Receptor are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 Receptor present in the samples.\u003cbr\u003e\nThis kit will recognize both endogenous and recombinant Human IL-6 Receptor.\u003cbr\u003e\nGet higher sensitivity in only 90 minutes with Human IL-6 Receptor ELISA Kit (\u003cbr\u003e\nab181424\u003cbr\u003e\n) from our SimpleStep ELISA\u003cbr\u003e\nrange.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nIL-6R also known as the IL-6 receptor or IL-6R protein is a transmembrane protein with an approximate mass of 80-90 kDa. This receptor is found on the surface of various cells including hepatocytes leukocytes and immune cells like T-cells and macrophages. IL-6R binds to interleukin-6 (IL-6) a cytokine and plays an important role in immune response regulation. The receptor exists in both membrane-bound and soluble forms each contributing to diverse signaling events.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIL-6 receptor function involves mediating the effects of IL-6 through its interaction with glycoprotein 130 (gp130) forming a signaling complex. This complex initiates intracellular pathways that lead to gene expression changes necessary for immune responses inflammation and hematopoiesis. When IL-6 binds IL-6R the complex formation with gp130 triggers downstream signaling that impacts cell growth differentiation and survival.\u003cbr\u003e\nPathways\u003cbr\u003e\nIL-6R is central to the JAK\/STAT signaling pathway one of the two main pathways activated by IL-6 signaling. It's also involved in the MAPK cascade contributing to cellular responses such as proliferation and differentiation. Through these pathways IL-6R interacts with other proteins like JAK kinases and STAT transcription factors coordinating cellular responses to external signals.\u003cbr\u003e\nIL-6R is associated with inflammatory diseases such as rheumatoid arthritis and certain cancers. In rheumatoid arthritis overexpression of IL-6R leads to persistent inflammation and joint damage. In cancer IL-6\/IL-6R signaling promotes tumorigenesis and cancer cell survival through interactions with proteins like STAT3. Understanding these connections aids in developing therapeutic interventions targeting IL-6R in various pathological conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843563737257,"sku":"ab46029","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab46029","provider":"Iright","version":"1.0","type":"link"}