{"product_id":"abcam-ab48506","title":"Abcam, ab48506, Anti-4 Hydroxynonenal antibody [HNEJ-2]","description":"\u003cp\u003eSize: 50µg\u003cbr\u003e\nAnti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506) is a mouse monoclonal antibody detecting 4 Hydroxynonenal in  Western Blot, IHC-P . - Over 200 publications - Trusted since 2007\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:HNEJ-2,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nApplications:IHC-FoFr, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506) is a mouse monoclonal antibody and is validated for use in IHC-FoFr, IHC-P and WB.\u003cbr\u003e\nAnti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506) was first used in a scientific publication in 1995 and has been cited over 203 times in peer reviewed journals. It's performance in Western blot and IHC in human, mouse and rat samples is trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality validation processes ensure Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506) has high sensitivity and specificity.\u003cbr\u003e\nAnti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506) has 9 independent reviews from customers.\u003cbr\u003e\nAnti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506) specifically detects 4 Hydroxynonenal (UniProt ID: N\/A; Molecular weight: 0.1kDa) and is sold in 50 µg selling sizes.\u003cbr\u003e\nTop cited antibody on the market with \u0026gt;302 citations and \u0026gt;10 reviews. 4-Hydroxynonenal is essential for detecting oxidative stress markers in cells, aiding research on diseases related to oxidative damage\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from serum-free cell culture medium by ammonium sulfate purification followed by protein A purification., Storage buffer-pH: 7.4Constituents: 0.115% Disodium hydrogenorthophosphate, 0.02% Potassium phosphate monobasic, 0.02% Potassium chloride, 0.0125% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\n4-Hydroxynonenal (4-HNE) often referred to as HNE is a highly reactive aldehyde with a molar mass of approximately 156.19 g\/mol. It plays a significant role in lipid peroxidation processes. 4-HNE forms during the oxidative degradation of polyunsaturated fatty acids and is present mostly in tissues with high metabolic activity. It is frequently expressed in the liver brain and heart where oxidative stress is elevated. Researchers commonly use techniques like 4-HNE staining and 4-HNE Western blot to study its presence and implications in various tissues and cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\n4-HNE serves as a signaling molecule influencing many cellular pathways. It is not just a byproduct of oxidative stress but actively modifies proteins through covalent binding. These modifications often lead to changes in protein function and structure affecting cellular processes such as proliferation apoptosis and differentiation. 4-HNE also interacts with other reactive molecules and antioxidants forming a complex network of cellular responses. This aldehyde regulates gene expression influencing how cells respond to oxidative stress and inflammation.\u003cbr\u003e\nPathways\u003cbr\u003e\n4-HNE interacts with pathways involved in oxidative stress and detoxification. In the oxidative stress pathway it reacts with glutathione to form glutathione conjugates which are part of the cell's detoxifying responses. The aldehyde thereby affects the Keap1-Nrf2-ARE pathway which modulates antioxidant responses and cellular protection against oxidative damage. Proteins like glutathione peroxidase and glutathione-S-transferase play key roles in these pathways illustrating the interconnectedness of 4-HNE with cellular defense mechanisms.\u003cbr\u003e\n4-HNE has associations with conditions such as Alzheimer's disease and cardiovascular diseases. In Alzheimer's the aldehyde contributes to neuroinflammation and protein modification impacting neuronal function and survival. It interacts with proteins like tau and amyloid-beta which are critical in the pathology of Alzheimer's. In cardiovascular diseases 4-HNE mediates cellular damage and inflammation linking it to the progression of atherosclerosis. This reactive aldehyde interacts with proteins such as apolipoprotein B highlighting its significant role in disease pathways linked to oxidative stress.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855758774441,"sku":"ab48506","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab48506","provider":"Iright","version":"1.0","type":"link"}