{"product_id":"abcam-ab52894","title":"Abcam, ab52894, Anti-EGFR antibody [EP38Y]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-EGFR antibody [EP38Y] (ab52894) is a rabbit monoclonal antibody detecting EGFR in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF, ELISA . Suitable for  Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 340 publications - Trusted since 2007\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EP38Y,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), IP, IHC-P, WB, ICC\/IF, I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:The immunogen for this product is a synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGFR. After screening, clone EP38Y was found to recognize total EGFR and is not specific to phosphorylated-Tyr1068 EGFR. This product yielded a strong signal in western blot using A431 (human squamous carcinoma) lysate which naturally overexpresses the EGFR protein. Western blot conditions may need to be optimised for cell lines and tissues that express lower levels of endogenous EGFR.The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-EGFR antibody [EP38Y] (ab52894) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), I-ELISA, ICC\/IF, IHC-P, IP and WB.\u003cbr\u003e\nAnti-EGFR antibody [EP38Y] (ab52894) was first used in a scientific publication in 2010 and has been cited over 342 times in peer reviewed journals. It's performance in Western Blot and IHC in human samples is trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-EGFR antibody [EP38Y] (ab52894) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nThe specificity of Anti-EGFR antibody [EP38Y] (ab52894) has been confirmed by Western Blot testing in EGFR knockout HeLa cells (\u003cbr\u003e\nab255385\u003cbr\u003e\nAnti-EGFR antibody [EP38Y] (ab52894) has 22 independent reviews from customers.\u003cbr\u003e\nAnti-EGFR antibody [EP38Y] (ab52894) specifically detects EGFR (UniProt ID: P00533; Molecular weight: 133kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone EP38Y -\u003cbr\u003e\nab174481\u003cbr\u003e\nAntibody clone EP38Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor\u003cbr\u003e\n647, Alexa Fluor\u003cbr\u003e\n488, HRP, Alexa Fluor\u003cbr\u003e\n594, PE, Alexa Fluor\u003cbr\u003e\n555, Biotin, Alexa Fluor\u003cbr\u003e\nab192982\u003cbr\u003e\nab193244\u003cbr\u003e\nab193602\u003cbr\u003e\nab207870\u003cbr\u003e\nab208753\u003cbr\u003e\nab274892\u003cbr\u003e\nab314262\u003cbr\u003e\nab321669\u003cbr\u003e\nThe epidermal growth factor receptor (EGFR) is a critical player in oncology due to its role in cell proliferation, survival and differentiation. It is a receptor tyrosine kinase that, when mutated or overexpressed, can drive the development and progression of various cancers, including non-small cell lung cancer (NSCLC) and glioblastoma.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.\u003cbr\u003e\nPathways\u003cbr\u003e\nEGFR is a central player in the MAPK and PI3K\/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.\u003cbr\u003e\nEGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844025766057,"sku":"ab52894","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab52894","provider":"Iright","version":"1.0","type":"link"}