{"product_id":"abcam-ab59569","title":"Abcam, ab59569, Human MICA ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 2 x 96Tests\u003cbr\u003e\nHuman MICA ELISA Kit is a sandwich ELISA designed to quantify Human MICA with a sensitivity of 123 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 156.25 - 5000 pg\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture supernatant,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 123 pg\/mL,\u003cbr\u003e\nRange:156.25 - 5000 pg\/mL,\u003cbr\u003e\nAssay time:3h 45m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman MICA ELISA Kit ab59569 is a sandwich ELISA to measure Human MICA in cell culture supernatant with a sensitivity of 123 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nA monoclonal antibody specific for MICA has been coated onto the wells of the microtiter strips provided. Samples, including standards of known MICA concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for MICA are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of MICA present in the samples.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman MICA ELISA Kit ab59569 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used\u003cbr\u003e\n2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature\u003cbr\u003e\n3. Aspirate and wash each well\u003cbr\u003e\n4. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature\u003cbr\u003e\n5. Aspirate and wash each well.\u003cbr\u003e\n6. Add the TMB Solution to each well until color develops and then add the Stop Solution\u003cbr\u003e\n7. Immediately begin recording the color development\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMICA also known as MHC class I polypeptide-related sequence A is a protein encoded by the MICA gene. It has a molecular weight of approximately 62 kDa. MICA is a stress-induced ligand expressed on the surface of various cell types including epithelial cells fibroblasts and some tumor cells. This protein is part of the MHC class I chain-related (MIC) family which plays an important role in the immune system by interacting with natural killer (NK) cells and certain T cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe MICA protein acts as a ligand for the NKG2D receptor an activating receptor found on NK cells and some T-cell subsets. MICA does not require expression as part of a complex with β2-microglobulin for function. When cells express MICA they signal distress allowing immune cells to target and destroy potentially harmful cells such as those infected with viruses or transformed into cancer cells. This interaction is an important component of the immune surveillance system.\u003cbr\u003e\nPathways\u003cbr\u003e\nMICA functions within the natural cytotoxicity effector pathway heavily involved in immune responses. It is also relevant in the DNA damage response pathways. Within these pathways MICA interacts with proteins like NKG2D and plays a part in mediating cellular responses to stress and damage. These interactions enhance the capacity of immune cells to recognize and eliminate abnormal cells.\u003cbr\u003e\nMICA is associated with conditions such as psoriasis and certain types of cancer. Studies have shown increased levels of soluble MICA in the sera of patients with psoriasis indicating a role in inflammation. Additionally in cancer MICA expression affects tumor immunity connecting to the NKG2D receptor to influence immune cell targeting and destruction of tumor cells. Researchers use tools such as MICA ELISA kits and imaging techniques to study its role in these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843646705833,"sku":"ab59569","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab59569","provider":"Iright","version":"1.0","type":"link"}