{"product_id":"abcam-ab64230","title":"Abcam, ab64230, Mounting Medium for IHC - Aqueous","description":"\u003cp\u003eSize: 125mL\u003cbr\u003e\nAqueous Mounting medium (ab64230) is suitable for chromogenic tissue and cell staining in IHC applications for brightfield microscopy. Compatible with most HRP \/ peroxidase and alkaline phosphatase (AP) enzymatic substrates. - Works with both aqueous and non-aqueous chromogens (e.g., Fast-Red, AEC, DAB) - No coverslip necessary- forms a dry, even surface for direct visualization\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:Preservative: 0.1% Sodium azide\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMounting Medium ab64230 is an aqueous mounting medium designed for use with chromogenic tissue and cell staining. It can be used with chromogens such as Fast-Red or AEC that require an aqueous mounting medium, or those that do not, such as DAB. When it is dry, it generates an even surface and can be used without a glass coverslip.\u003cbr\u003e\nMounting media products\u003cbr\u003e\nFor fluorescent cell and tissue staining, Abcam recommends aqueous, anti-fade\u003cbr\u003e\nFluorescence Mounting Medium ab104135\u003cbr\u003e\nMounting Medium with DAPI ab104139\u003cbr\u003e\nMounting Medium with PI ab104129\u003cbr\u003e\n. For thick sections or tissues containing lots of fat, we recommend\u003cbr\u003e\nGlycerol Mounting Medium with DAPI ab188804\u003cbr\u003e\nFor chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend this product, Mounting Medium ab64230, or organic\u003cbr\u003e\nLimonene Mounting Medium ab104141\u003cbr\u003e\nIHC protocol suitable for use with Mounting Medium ab64230:\u003cbr\u003e\n1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.\u003cbr\u003e\n2. Use appropriate\u003cbr\u003e\nantigen retrieval buffer or enzyme\u003cbr\u003e\n(primary antibody dependent) to treat sections. Wash 3 times in buffer.\u003cbr\u003e\n3. Add enough\u003cbr\u003e\nhydrogen peroxide blocking solution\u003cbr\u003e\nto cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use\u003cbr\u003e\navidin biotin blocking\u003cbr\u003e\n4. Apply\u003cbr\u003e\nprotein block\u003cbr\u003e\nnormal serum\u003cbr\u003e\nfrom same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.\u003cbr\u003e\n5. Apply primary antibody in\u003cbr\u003e\nantibody diluent\u003cbr\u003e\nand incubate.\u003cbr\u003e\n6. Wash 4 times in buffer. Incubate slide with\u003cbr\u003e\nbiotinylated secondary antibody\u003cbr\u003e\nHRP polymer secondary antibody\u003cbr\u003e\nand skip step 7). Wash 4 times in buffer.\u003cbr\u003e\n7. Apply\u003cbr\u003e\nstreptavidin-HRP\u003cbr\u003e\nand incubate for 10 minutes at room temperature.\u003cbr\u003e\n8. Rinse 4 times in buffer. Place slide in\u003cbr\u003e\nDAB substrate\u003cbr\u003e\nAEC Substrate\u003cbr\u003e\nand incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.\u003cbr\u003e\n9. Add enough drops of\u003cbr\u003e\nhematoxylin\u003cbr\u003e\nto cover the section. Incubate for 1 minute.\u003cbr\u003e\n10. Rinse 7-8 times in tap water. Add Mounting Medium ab64230 to cover the section.\u003cbr\u003e\nFind complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the\u003cbr\u003e\nIHC kits and reagents guide\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847573196969,"sku":"ab64230","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab64230","provider":"Iright","version":"1.0","type":"link"}