{"product_id":"abcam-ab65302","title":"Abcam, ab65302, Cathepsin D Activity Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 100Test \/ 2000Test\u003cbr\u003e\nCathepsin D Activity Assay Kit (Fluorometric) ab65302 uses a substrate based on the GKPILFFRLK(Dnp)-D-R-NH2) Cathepsin D substrate. Add reaction mix to samples and incubate for 1-2 hr at 37°C. Readout on any fluorometric (Ex\/Em 328\/460 nm) plate reader. - Cited in \u0026gt; 120 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Tissue Extracts, Cell Lysate,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nAssay time:2h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCathepsin D Activity Assay Kit (Fluorometric) ab65302 is a fluorescence-based assay to measure Cathepsin D.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nCathepsin D Activity Assay Kit (Fluorometric) utilizes the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2) labeled with MCA.\u003cbr\u003e\nCell lysates or other samples that contain cathepsin-D will cleave the synthetic substrate to release fluorescence, which can then easily be quantified using a fluorometer or fluorescence plate reader at Ex\/Em = 328\/460 nm.\u003cbr\u003e\nCathepsin D assay protocol summary:\u003cbr\u003e\nAdd samples to wells\u003cbr\u003e\n- Add reaction mix and incubate for 1-2 hr at 37°C\u003cbr\u003e\n- Measure fluorescence (Ex\/Em = 328\/460 nm)\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nSee below other alternative Cathepsin Assay Kits:\u003cbr\u003e\n- Cathepsin L Assay Kit (Magic Red)\u003cbr\u003e\nab270774\u003cbr\u003e\n- Cathepsin K Assay Kit (Magic Red)\u003cbr\u003e\nab270774\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K143 Cathepsin D Activity Fluorometric Assay Kit. K143-100 is the same size as the 100 test size of ab65302.\u003cbr\u003e\nApoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCathepsin D also known as CTSD is a protein with a mass of approximately 45 kDa. It functions as an aspartyl protease and is expressed in lysosomes across various tissues including the liver and kidneys. This enzyme acts by cleaving peptide bonds within proteins which is essential for protein degradation and turnover. Cathepsin D exists as precursor forms that become activated in the acidic environment of the lysosome. It plays a critical role in normal cellular processes by maintaining protein homeostasis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzymatic activity of Cathepsin D is important for cellular maintenance and apoptosis. This protease does not act within larger protein complexes but contributes to the degradation of extracellular and intracellular proteins. It mediates processes like antigen processing where it deconstructs proteins into peptides that are presented on major histocompatibility complex (MHC) molecules. ELISA tests can quantify its expression levels sometimes termed as CTSD activity in various biological samples offering insights into its role within cellular environments.\u003cbr\u003e\nPathways\u003cbr\u003e\nCathepsin D involvement includes the lysosomal degradation pathway and the apoptotic signaling pathway. In the lysosomal degradation pathway Cathepsin D breaks down proteins and peptides a process important for cellular recycling and energy release. It interacts with other lysosomal enzymes such as Cathepsin B in this pathway ensuring comprehensive breakdown of cellular waste. The apoptotic signaling pathway involves the regulation of programmed cell death where Cathepsin D can influence the activation of downstream proteins like Bcl-2 and Bax which control cell survival.\u003cbr\u003e\nThe overexpression of Cathepsin D links to breast cancer and Alzheimer's disease. In breast cancer increased Cathepsin D expression correlates with tumor progression and metastasis influencing tumor behavior through interactions with other proteins involved in cell proliferation. Alzheimer's disease features the involvement of Cathepsin D in the breakdown of amyloid precursor protein which relates to amyloid beta plaque accumulation. The abnormal activity of Cathepsin D in these disorders makes it a potential target for therapeutic antibodies such as CTSD antibodies which aim to regulate its function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843541618857,"sku":"ab65302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab65302","provider":"Iright","version":"1.0","type":"link"}