{"product_id":"abcam-ab65337","title":"Abcam, ab65337, Free Glycerol Assay Kit","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nGlycerol Assay Kit (ab65337) provides a sensitive, easy assay to quantify free glycerol concentration. Readout on any colorimetric (570 nm) or fluorometric (Ex\/Em 535\/587 nm) plate reader. - Cited in over 35 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric\/Fluorometric,\u003cbr\u003e\nSample types:Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026gt; 1 µM,\u003cbr\u003e\nRange:1 - 10000 µM,\u003cbr\u003e\nAssay time:40m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nGlycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration.\u003cbr\u003e\nIn the glycerol assay protocol, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex\/Em = 535\/587 nm).\u003cbr\u003e\nThis glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 μM range) of glycerol.\u003cbr\u003e\nGlycerol assay protocol summary:\u003cbr\u003e\n- add samples and standards to wells\u003cbr\u003e\n- add reaction mix and incubate for 30 min\u003cbr\u003e\n- analyze with a microplate reader\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K630 Free Glycerol Colorimetric\/Fluorometric Assay Kit. K630-100 is the same size as the 100 test size of ab65337.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlycerol also known as glycerol anhydrous or glycérol is a simple polyol compound with a molecular weight of approximately 92.09 g\/mol. Glycerol is expressed widely in various tissues and plays a considerable role as a backbone in the structure of triglycerides and phospholipids thereby impacting lipid biology significantly. It acts mechanically in lipid metabolism by serving as a substrate for glycerolipid synthesis and provides energy through its conversion to glucose or dihydroxyacetone phosphate.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGlycerol metabolism is significant in energy storage and lipid regulation. It does not function in isolation but is part of various lipid complexes that regulate lipid homeostasis. Through its involvement it aids in the formation of essential lipid components that are important for membrane structure and function. The ability of cells to store energy efficiently is largely dependent on glycerol's capability to integrate into and support these lipid structures.\u003cbr\u003e\nPathways\u003cbr\u003e\nGlycerol participates in glycerolipid metabolism and gluconeogenesis. The glycolytic pathway is especially important as glycerol serves as a potential precursor for glucose production connecting it to energy supply chains within the organism. Proteins like glycerol kinase and glycerol-3-phosphate dehydrogenase play critical roles in these conversions and metabolic processes underlying how glycerol fits into broader biological functions.\u003cbr\u003e\nGlycerol has connections to metabolic conditions such as glycerol kinase deficiency and nonalcoholic fatty liver disease. Its metabolism's rate and efficiency can influence lipid accumulation and related metabolic syndromes. The enzyme glycerol kinase which phosphorylates glycerol to form glycerol-3-phosphate associates directly with glycerol's relevance in these diseases. Understanding these connections may lead to insights into managing or alleviating related metabolic disease symptoms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843610595497,"sku":"ab65337","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab65337","provider":"Iright","version":"1.0","type":"link"}