{"product_id":"abcam-ab692","title":"Abcam, ab692, Anti-Bcl-2 antibody [100\/D5]","description":"\u003cp\u003eSize: 500µL\u003cbr\u003e\nMouse Monoclonal Bcl-2 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC\/IF and reacts with Human, Cat, Horse, Pig, Llama samples. Cited in 304 publications. Immunogen corresponding to Synthetic Peptide within Human BCL2 aa 1-100.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:100\/D5,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human, Cat, Horse, Llama, Pig,\u003cbr\u003e\nApplications:IHC-P, WB, Flow Cyt, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human BCL2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.P10415\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThis product was changed from ascites to tissue culture supernatant on 8\u003cbr\u003e\nMarch 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.3Preservative: 0.09% Sodium azideConstituents: Tissue culture supernatant, PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nBcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.\u003cbr\u003e\nPathways\u003cbr\u003e\nBcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.\u003cbr\u003e\nThe dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844230664361,"sku":"ab692","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab692","provider":"Iright","version":"1.0","type":"link"}