{"product_id":"abcam-ab7315","title":"Abcam, ab7315, Anti-pan methyl Lysine antibody - ChIP Grade","description":"\u003cp\u003eSize: 50µg\u003cbr\u003e\nRabbit Polyclonal H3 antibody. Suitable for IP, ChIP, ELISA, WB, IHC-P, ICC\/IF and reacts with Modified Amino Acid samples. Cited in 33 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nApplications:WB, IP, ELISA, ChIP, ICC\/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:ab7315 recognises Histone H3 di-methyl K4, di-methyl K9 and di-methyl K27 in WB. Non-histone samples have not been tested, thus, we do not know if ab7315 would work on non-histone samples.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThis antibody will be extremely useful in the study of the regulation of transcription by methylation. Has also been successfully used in CHIP in both human and yeast.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Add glycerol to a final volume of 50% for extra stability and aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPan methyl Lysine a common post-translational modification involves the methylation of lysine residues on histones and other proteins. This modification is a marker for chromatin states and gene expression regulation. Pan methyl Lysine does not refer to a single protein but rather a type of modification found on various proteins impacting their function. Any measurement of mass refers to the specific protein that bears this modification. You can find pan methyl Lysine in the cell nucleus of eukaryotic organisms where it plays a role in gene expression regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe methylation of lysine residues affects processes like transcription replication and DNA repair. It interacts with chromatin-modifying complexes and transcription factors. This modification can facilitate or inhibit the binding of proteins to chromatin thereby influencing gene expression. In addition pan methyl Lysine can undergo mono- di- or tri-methylation each with distinct biological impacts on protein function and cellular processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nMechanisms involving lysine methylation tie closely with epigenetic regulation pathways alongside histone acetylation phosphorylation and ubiquitination. Proteins such as histone methyltransferases and demethylases regulate the methylation status of lysine residues affecting chromatin dynamics and consequently gene expression. Methyl lysine modifications can work in concert with other histone modifications within the histone code pathway dictating specific gene expression patterns in response to cellular signals.\u003cbr\u003e\nAbnormalities in lysine methylation link to cancer and neurological disorders. Alterations in methylation patterns can lead to dysregulation of oncogenes or tumor suppressor genes contributing to cancer progression. Proteins like mixed lineage leukemia (MLL) and enhancer of zeste homolog 2 (EZH2) which involve lysine methylation often show mutations or misregulation in cancer-related pathways. In neurological conditions abnormal methylation affects gene expression patterns important for neural function and development linking it to disorders such as schizophrenia.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850100297897,"sku":"ab7315","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab7315","provider":"Iright","version":"1.0","type":"link"}