{"product_id":"abcam-ab7403","title":"Abcam, ab7403, Streptavidin (HRP)","description":"\u003cp\u003eSize: 500µg\u003cbr\u003e\nStreptavidin (HRP) is a Full Length native Streptavidin protein expressed in Streptomyces avidinii and conjuated with Horseradish peroxidase (HRP). ab7403 has \u0026gt;95% purity and suitable for western blot, ELISA, IHC, ICC, Dot blot and more. - Used to detect biotin-conjugated primary antibodies - Has less non-specific binding and less background signal compared to avidin - Cited in over 170 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nExpression system:Native,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:ELISA, WB, ICC, IHC-P, IHC-Fr, IM, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Binds to Biotin. Dissociation constant has not been measured.,\u003cbr\u003e\nConjugation:HRP,\u003cbr\u003e\nAccession:P22629,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Streptomyces avidinii,\u003cbr\u003e\nStorage buffer:Preservative: 0.01% Gentamicin sulphateConstituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEnsure the validity of your result using our Streptavidin (HRP) ab7403 to detect biotinylated primary antibodies in western blot, ELISA, IHC and more.\u003cbr\u003e\nCheck out our ELISA protocol for more information\u003cbr\u003e\nHorseradish peroxidase is conjugated to the streptavidin tetramer at ~1:1 molar ratio.\u003cbr\u003e\nThis product has been assayed against 1.0 μg of Biotinylated IgG in a standard capture ELISA using the peroxidase substrate ABTS (\u003cbr\u003e\nab142041\u003cbr\u003e\n) as a substrate for 30 minutes at room temperature.\u003cbr\u003e\nA working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.\u003cbr\u003e\nOptimal titers for other applications should be determined by the researcher.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843797274793,"sku":"ab7403","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab7403","provider":"Iright","version":"1.0","type":"link"}