{"product_id":"abcam-ab7914","title":"Abcam, ab7914, HL-60 whole cell lysate","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nHL-60 whole cell lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HL-60,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nForm:LiquidSee storage information\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCell line: HL60 (Human promyelocytic leukemia).\u003cbr\u003e\nGrowth media: RPMI and 10% FBS (Fetal bovine serum).\u003cbr\u003e\nHL60 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg\/ml of aprotinin, 5 µg\/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845055336617,"sku":"ab7914","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab7914","provider":"Iright","version":"1.0","type":"link"}