{"product_id":"abcam-ab8112","title":"Abcam, ab8112, Anti-DOK1 antibody","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRabbit Polyclonal DOK1 antibody. Suitable for WB, ICC\/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human Docking protein 1 aa 400-450.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human Docking protein 1 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.Q99704\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nTyrosine kinase substrate p62DOK\u003cbr\u003e\nSignals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDOK1 also known as Docking protein 1 or Downstream of tyrosine kinase 1 is an adaptor protein involved in the signal transduction pathways. This protein weighs approximately 62 kDa and is primarily expressed in hematopoietic cells. While DOK1 lacks enzymatic activity it plays an important role in cellular processes by providing a scaffold for various signaling molecules. It helps to modulate downstream signaling events by interacting with receptor tyrosine kinases and other proteins through its multiple binding domains.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDOK1 operates as a negative regulator in signal transduction functioning as part of a larger signaling complex. The protein associates with different cellular components forming interactions that influence cell communication and immune response. DOK1 often interacts with proteins like RasGAP and Nck which helps in mediating signals that can affect cell growth differentiation and apoptosis. Its role as a docking station for other proteins allows it to influence cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nDOK1 influences the Ras and MAP kinase signaling pathways. It inhibits the Ras pathway by binding with and recruiting RasGAP leading to the inactivation of Ras an important player in cell proliferation. Additionally DOK1 modulates the JAK\/STAT pathway by affecting cytokine signaling and immune regulation. These interactions are significant for controlling cellular responses and maintaining balance in cell signaling networks.\u003cbr\u003e\nDOK1 is implicated in conditions like leukemia and autoimmune diseases. Aberrant DOK1 expression or malfunction disrupts normal signal transduction contributing to the pathogenesis of these disorders. In leukemia altered DOK1 activity can affect its interactions with proteins such as RasGAP leading to unchecked cell growth and survival. Similarly in autoimmune diseases DOK1's regulatory effect on JAK\/STAT signaling might be compromised impacting immune cell function and response.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855795409065,"sku":"ab8112","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab8112","provider":"Iright","version":"1.0","type":"link"}