{"product_id":"abcam-ab8284","title":"Abcam, ab8284, Anti-Histone H3 (asymmetric di methyl R17) antibody","description":"\u003cp\u003eSize: 50µg\u003cbr\u003e\nRabbit Polyclonal H3 asymmetric di methyl R17 antibody. Suitable for Dot, WB, PepArr, IHC-P, ICC\/IF and reacts with Synthetic peptide, Human, Synthetic peptide - Human samples. Cited in 68 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:PepArr, IHC-P, ICC\/IF, Dot, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:Peptide competition experiments confirmed that the antibody recognises specifically methylated R17 in H3 and not unmethylated H3 or methylated R3 in H4 (see figure 1). In whole cell extract the antibody recognises specifically only the methylated histone H3 protein band (see figure 2) Further, the antibody doesn't crossreact with the C-terminal methylation sites of CARM1 in histone H3. In IHC on paraffin-embedded sections of human tonsil, the antibody shows nuclear staining across most nuclei. Slight batch to batch variation is observed, but no more than 50% cross reactivity with symmetric di methyl R17 peptide is allowed.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThe nuclear hormone receptor co-activator CARM1 has the potential to methylate histone H3 at arginine residues in vitro. The methyltransferase activity of CARM1 is necessary for its co-activator functions in transient transfection assays. However, the role of this methyltransferase in vivo is unclear, given that methylation of arginines is not easily detectable on purified histones. This antibody recognizes methylated arginine 17 (R17) of histone H3, the major site of methylation by CARM1. Bauer et al (2001) have shown by using this antibody that methylated R17 exists in vivo. Chromatin immunoprecipitation analysis shows that R17 methylation on histone H3 is dramatically upregulated when the estrogen receptor-regulated pS2 gene is stimulated by estradiol and TPA. Coincident with the appearance of methylated R17, the CARM1 methyltransferase is found associated with the histones on the pS2 gene. Together these results demonstrate that the CARM1 methyltransferase is recruited to an active promoter and that CARM1-mediated methylation of histone H3 at R17 takes place in vivo during this active state.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847791300777,"sku":"ab8284","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab8284","provider":"Iright","version":"1.0","type":"link"}