{"product_id":"abcam-ab83205","title":"Abcam, ab83205, Recombinant Human Rb protein","description":"\u003cp\u003eSize: 5µg\u003cbr\u003e\nRecombinant Human Rb protein is a Human Full Length protein, in the 1 to 928 aa range, expressed in Baculovirus, with \u0026gt;95% purity and suitable for western blot, SDS-PAGE, GSA and EMSA.The predicted molecular weight of ab83205 protein is 107.5 kDa. - Save time and ensure accurate results- use our Retinoblastoma protein (Rb) as a control\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nExpression system:Baculovirus,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, WB, GSA, EMSASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P06400,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 7.9Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEnsure the validity of your result using our recombinant human Retinoblastoma protein (Rb\/Rb1\/pRb) protein ab83205 as a positive control in western blot and SDS-PAGE.\u003cbr\u003e\nThe Rb protein ab83205: 1 nanogram of purified protein is sufficient for a gel mobility shift assay (GSA) in a 20 µl reaction; 50 nanogram are sufficient for reconstituted transcription assay and 100 nanogram units are sufficient for a protein-protein interaction assay\u003cbr\u003e\nCheck out our protein gel staining guide for SDS-PAGE\u003cbr\u003e\nCheck out of western blot protocol for more information\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRetinoblastoma protein (Rb) also known as pRb is an important regulatory protein with a molecular weight of approximately 110 kDa. It is mainly expressed in the nucleus of cells. Rb functions as a tumor suppressor by controlling the cell cycle progression from G1 to S phase. Rb becomes active when it is dephosphorylated allowing it to bind and inhibit E2F transcription factors consequently preventing the transcription of genes essential for S phase entry.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRetinoblastoma protein influences cellular proliferation differentiation and apoptosis. It acts within a larger protein complex modulating various cellular responses. When functional Rb halts uncontrolled cell division important for maintaining normal tissue homeostasis. In its phosphorylated state known as phospho-Rb or phospho-Rb E182 it loses its regulatory capabilities which can lead to unrestrained cell cycle progression.\u003cbr\u003e\nPathways\u003cbr\u003e\nSeveral involve the retinoblastoma protein. One key pathway is the p53 pathway which Rb interacts with to influence cellular outcomes like cell-cycle arrest and apoptosis. Rb cooperates with proteins like p21 to implement these processes. Additionally it is involved in the cyclin D-dependent kinase 4 (CDK4) pathway which modulates its phosphorylation state influencing binding interactions and cell cycle control.\u003cbr\u003e\nDysregulation of retinoblastoma protein is commonly associated with cancer particularly retinoblastoma and breast cancer. In retinoblastoma mutations in the Rb gene directly lead to uncontrolled cell proliferation due to the absence of functional Rb protein. Additionally Rb's connection to the E2F family of transcription factors can become disrupted contributing to oncogenesis in other cancers.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843813331113,"sku":"ab83205","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab83205","provider":"Iright","version":"1.0","type":"link"}