{"product_id":"abcam-ab83371","title":"Abcam, ab83371, Alkaline Phosphatase Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 500Test\u003cbr\u003e\nAlkaline Phosphatase Assay Kit ab83371 is an addition-only ALP activity assay with just one 30 min incubation step. Readout on any fluorometric plate reader (Ex\/Em 360\/440 nm). - Cited in over 40 publications -Simple and fast protocol with results in one hour - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Saliva, Urine (UTI), Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAlkaline Phosphatase Assay Kit (Fluorometric) (ab83371) is a highly sensitive kit to detect alkaline phosphatase (ALP) activity in serum and other biological samples. In the alkaline phosphatase assay protocol, ALP cleaves the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (MUP) substrate, resulting in an increased fluorescent signal (Ex\/Em = 360nm\/440nm) when the substrate is dephosphorylated.\u003cbr\u003e\nThe kit is an ultra sensitive, simple, direct and HTS-ready assay designed to measure ALP activity with a detection sensitivity ~1 μU, more sensitive than colorimetric assays.\u003cbr\u003e\nAlkaline phosphatase assay protocol summary:\u003cbr\u003e\n- add samples and standards to wells\u003cbr\u003e\n- add reaction mix to wells\u003cbr\u003e\n- add ALP enzyme solution to standard wells (not to samples)\u003cbr\u003e\n- incubate for 30 min at room temp\u003cbr\u003e\n- add stop solution\u003cbr\u003e\n- analyze with microplate reader\u003cbr\u003e\nTechnical Note: ALP is unlikely to be detectable in healthy urine but may be elevated in the presence of infection.\u003cbr\u003e\nRelated alkaline phosphatase assay products\u003cbr\u003e\nFor a colorimetric alkaline phosphatase assay kit, we recommend\u003cbr\u003e\nab83369\u003cbr\u003e\n. which is based on the p-nitrophenyl phosphate (pNPP) assay substrate. pNPP turns yellow (405 nm) when dephosphorylated by ALP as part of an ALP activity assay.\u003cbr\u003e\nFor a luminescent alkaline phosphatase assay, we recommend\u003cbr\u003e\nab233466\u003cbr\u003e\n, which is based on the ability of alkaline phosphatase to remove the phosphate group from D-luciferin phosphate. This releases the luciferin to be processed by luciferase.\u003cbr\u003e\nFor alkaline phosphatase staining, we recommend Alkaline Phosphatase Staining Kit\u003cbr\u003e\nab284936\u003cbr\u003e\n, which is based on the substrate, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) substrate. BCIP is processed by alkaline phosphatase to form a blue colored intermediate. Nitro Blue Tetrazolium (NBT) then oxidizes the intermediate to form an insoluble, dark blue dimer, which can be viewed by microscopy.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K422 Alkaline Phosphatase Activity Fluorometric Assay Kit. K422-500 is the same size as the 500 test size of ab83371.\u003cbr\u003e\nAlkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. The change in alkaline phosphatase level and activity is associated with many diseases in the liver and bones.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlkaline phosphatase also known as ALP is an enzyme with significant biological roles. It mechanically functions by catalyzing the hydrolysis of phosphate groups from various molecules including nucleotides proteins and alkaloids under alkaline conditions. ALP is a metalloenzyme possessing zinc and magnesium ions at its active site to aid in the reaction. This enzyme has a molecular mass of approximately 86 kDa and exists in dimeric form. ALP is expressed in many tissues with high levels in liver bone kidney and the placenta.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nALP plays an important role in maintaining mineral balance and bone metabolism by breaking down pyrophosphate an inhibitor of mineralization. It facilitates the regulation of phosphate metabolism by contributing to phosphorus and calcium homeostasis. ALP is not part of a larger complex but interacts with calcium and phosphate ions to aid in the formation and resorption of bone mineral tissue. It serves as a marker in bone growth and liver function due to its prominent activity in those tissues visible in assays like the ALP staining method used to gauge enzyme presence and action.\u003cbr\u003e\nPathways\u003cbr\u003e\nAlkaline phosphatase activity plays an essential role in the metabolic pathways related to bone mineralization and liver function. In bone tissue the enzyme participates in the calcification process important for bone strength and development by regulating inorganic phosphate availability. The liver expression of ALP is involved in bile excretion pathways where it dephosphorylates bile acids aiding in their conversion and recycling. It has a significant relationship with proteins such as osteocalcin in the bone mineralization pathway and bile acid transporters in the liver.\u003cbr\u003e\nAlkaline phosphatase levels serve as diagnostic indicators for bone and liver diseases. High levels of ALP are often associated with bone disorders like Paget's disease and liver conditions like cholestasis. In these disorders ALP activity rises due to increased enzyme synthesis by osteoblasts in bone diseases or impaired bile flow in liver diseases. Furthermore the liver enzyme gamma-glutamyl transferase (GGT) is often measured alongside ALP to differentiate the cause of elevated ALP levels particularly to assess liver pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843609153705,"sku":"ab83371","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab83371","provider":"Iright","version":"1.0","type":"link"}