{"product_id":"abcam-ab94052","title":"Abcam, ab94052, Rad1 overexpression 293T lysate (whole cell)","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRad1 overexpression 293T lysate (whole cell) suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.\u003cbr\u003e\nKey facts\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nForm:LiquidSee storage information\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab94052 is a 293T cell transfected lysate in which Human Rad1 has been transiently over-expressed using a pCMV-Rad1 plasmid. The lysate is provided in 1X Sample Buffer.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRad1 also known as RAD1 homolog 1 from Saccharomyces cerevisiae functions mechanically as an exonuclease involved in DNA repair processes. It forms a part of the 9-1-1 checkpoint complex which acts as a sensor for DNA damage. Rad1 has a molecular mass of approximately 34 kDa and is expressed in various tissues where cell division occurs. Its primary role is in maintaining genomic stability by participating in the recognition and repair of abnormal DNA structures.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRad1 plays an essential role in the DNA damage response by being part of the 9-1-1 complex which includes Rad9 and Hus1 proteins. This complex is an important player in activating the ATR signaling pathway which helps cells respond to DNA replication stress and damage. Rad1 helps ensure proper checkpoint activation preventing cells with damaged DNA from progressing through the cell cycle which further safeguards genomic integrity.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe protein Rad1 functions critically within the ATR-Chk1 signaling pathway a significant route for controlling cell cycle checkpoints. In this context Rad1 associates with proteins such as ATR and Chk1 facilitating the signaling necessary for cell cycle arrest under conditions of DNA stress. Rad1 also interacts with the base excision repair pathway where it works with other repair proteins to rectify oxidative DNA damage and maintain cellular viability.\u003cbr\u003e\nRad1 has a connection with cancer due to its involvement in DNA repair pathways. Impaired Rad1 function can lead to accumulation of DNA damage contributing to carcinogenesis. Additionally Rad1’s participation in maintaining genomic stability links it to disorders like Fanconi anemia where DNA repair defects are evident. In these contexts Rad1's interactions with proteins like BRCA1 further highlight its importance in protective cellular mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845120184489,"sku":"ab94052","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab94052","provider":"Iright","version":"1.0","type":"link"}