{"product_id":"abcam-ab94221","title":"Abcam, ab94221, TSC-1 overexpression 293T lysate (whole cell)","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nTSC-1 overexpression 293T lysate (whole cell) suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.\u003cbr\u003e\nKey facts\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nForm:LiquidSee storage information\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab94221 is a 293T cell transfected lysate in which Human TSC-1 has been transiently over-expressed using a pCMV-TSC-1 plasmid. The lysate is provided in 1X Sample Buffer.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTuberous Sclerosis Complex 1 (TSC-1) also known as hamartin is a protein with a molecular mass of approximately 130 kDa. It plays a direct role in cellular signaling and regulatory functions. TSC-1 is expressed broadly across tissues which suggests its importance in maintaining normal cellular physiology. It interfaces with various signaling molecules and directly interacts with TSC-2 to form a protein complex.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTSC-1 plays a regulatory role in cell growth and proliferation. It forms a complex with TSC-2 also known as tuberin which is important for its function. This complex acts as a GAP (GTPase-activating protein) toward Rheb a small GTPase inhibiting the mTOR signaling pathway. The proper regulation of this pathway ensures controlled cell size and growth emphasizing the importance of the TSC-1\/TSC-2 complex in cellular regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nTSC-1 operates within the mTOR signaling pathway to influence cellular processes like autophagy and protein synthesis. It also relates to the PI3K\/AKT pathway one that is involved in the regulation of cell survival and metabolism. By interacting with TSC-2 in these pathways TSC-1 modulates the inhibition of mTORC1 thereby impacting cellular homeostasis and growth.\u003cbr\u003e\nDefects in TSC-1 can lead to tuberous sclerosis complex a condition characterized by benign tumors in multiple organs. Mutations in TSC-1 and its partner TSC-2 disrupt normal cellular functions leading to this disease. Additionally aberrations in the mTOR pathway influenced by TSC-1 malfunction have associations with certain cancers highlighting the importance of TSC-1 in maintaining cell proliferation and signaling balance.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845119987881,"sku":"ab94221","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab94221","provider":"Iright","version":"1.0","type":"link"}