{"product_id":"abcam-ab9465","title":"Abcam, ab9465, Anti-Sarcomeric Alpha Actinin antibody [EA-53]","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nAnti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) is a mouse monoclonal antibody detecting Sarcomeric Alpha Actinin in  Western Blot, IHC-P . Suitable for  Human, Rat . - Clone EA-53 is the most cited clone to Sarcomeric Alpha Actinin - Over 270 publications - Trusted since 2002\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EA-53,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:unknown,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Rat, Human,\u003cbr\u003e\nApplications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) is a House Mouse Monoclonal antibody and is validated for use in IHC-P, WB in human, mouse, rat samples.\u003cbr\u003e\nAnti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) has been cited over 277 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAbcams high quality validation processes ensure Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) has high sensitivity and specificity.\u003cbr\u003e\nAnti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) has 12 independent reviews from customers.\u003cbr\u003e\nAnti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) specifically detects Sarcomeric Alpha Actinin (UniProt ID: P35609; Molecular weight: 104kDa) and is sold in 100 uL selling sizes.\u003cbr\u003e\nSarcomeric Alpha Actinin is a crucial protein in the muscle cytoskeleton, specifically localized at the Z-disc. The alpha actinin molecular weight is approximately 100 kDa, and it plays a vital role in maintaining sarcomere integrity. The a-actinin (ACTN2) antibody is essential tools for studying this protein's function. Abnormalities in alpha actinin are linked to various muscle diseases, including hypertrophic cardiomyopathy and nemaline myopathy, highlighting its disease relevance.\u003cbr\u003e\nThis product was changed from ascites to tissue culture supernatant on 9\u003cbr\u003e\nSeptember 2019. Lot numbers higher than GR3258034 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.\u003cbr\u003e\nStorage in \"frost-free\" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Proprietary technique, Purification notes-Purified from tissue culture supernatant., Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSarcomeric Alpha Actinin often referred to as alpha-actinin or A-actinin is a protein that functions mechanically as a cross-linker of actin filaments in the sarcomere. It plays a critical role in organizing the actin cytoskeleton providing structural integrity and facilitating muscle contraction. The protein typically has a molecular mass of approximately 100 kDa. It is expressed in a variety of tissues including skeletal and cardiac muscle where it establishes the architecture of the Z-disc by anchoring actin filaments.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSarcomeric alpha actinin associates with multiple proteins to form a complex that coordinates muscle function. It not only serves as a structural component but also participates in signaling processes influencing muscle repair and growth. Alpha actinin connects to other cytoskeletal proteins and adaptors coordinating with integrin pathways to transmit external mechanical signals into cellular responses. The ability of alpha actinin to interact with a range of proteins like filamin and spectrin enhances its role in maintaining cellular architecture.\u003cbr\u003e\nPathways\u003cbr\u003e\nAlpha actinin is critical in both the integrin and dystrophin-glycoprotein complex pathways facilitating signal transduction and maintaining muscle stability. Its interaction with actin filaments is important for pathways involving cell adhesion and motility. Within these pathways actin-related proteins such as ACTN2 and titin partner with alpha actinin to sustain cellular structure and function during dynamic processes including muscle contraction and relaxation.\u003cbr\u003e\nMutations or defects in alpha actinin have implications in muscular dystrophy and cardiomyopathy. These conditions often involve disruptions in the muscle's structural framework and mechanical signaling where alpha actinin's interaction with dystrophin becomes significant. Additionally such disorders may showcase altered connectivity between alpha actinin and spectrin further affecting muscle integrity and function in pathological conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855755661481,"sku":"ab9465","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab9465","provider":"Iright","version":"1.0","type":"link"}