{"product_id":"abcam-ab9659","title":"Abcam, ab9659, Recombinant human Interferon gamma protein (Active)","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant human Interferon gamma protein (Active) is a Human Full Length protein, in the 1 to 144 aa range with \u0026gt;98% purity, \u0026lt; 1 EU\/µg endotoxin level and suitable for western blot, SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab9659 protein is 16.8 kDa. - Save time and ensure accurate results- use our IFN-gamma protein as a control - Optimal protein bioactivity and stability\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;98% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt; 1 EU\/µg,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:SDS-PAGE, HPLC, WB, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:The ED50 determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng\/ml, corresponding to a specific activity of ≥ 2 x 10^7 units\/mg.,\u003cbr\u003e\nAccession:P01579,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:reconstitute with PBS, pH 8.0 at 100µL,\u003cbr\u003e\nStorage buffer:Constituents: PBS\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEnsure the validity of your result using our recombinant bioactive human IFN-gamma protein ab9659 as a positive control in western blot, SDS-PAGE and HPLC. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation.\u003cbr\u003e\nHuman IFN gamma does not show cross-reactivity with mouse.\u003cbr\u003e\nCheck out our protein gel staining guide for SDS-PAGE\u003cbr\u003e\nCheck out of western blot protocol for more information\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nInterferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naпve T cells into Th1 cells which is essential for effective cellular immunity.\u003cbr\u003e\nPathways\u003cbr\u003e\nIFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.\u003cbr\u003e\nIFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843805958313,"sku":"ab9659","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab9659","provider":"Iright","version":"1.0","type":"link"}