{"product_id":"abcam-ab99970","title":"Abcam, ab99970, Human Angiogenin ELISA Kit (ANG)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Angiogenin ELISA Kit (ANG) is a Sandwich (quantitative) ELISA for the measurement of Human Angiogenin (ANG) in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 1.5 pg\/mL,\u003cbr\u003e\nRange:1.64 - 400 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Angiogenin (ANG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of Human Angiogenin in serum, plasma and cell culture supernates.\u003cbr\u003e\nThis assay employs an antibody specific for Human Angiogenin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiogenin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiogenin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiogenin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nOptimisation may be required with urine samples.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAngiogenin also known as ANG is a small protein with a molecular mass of approximately 14 kDa. It belongs to the ribonuclease A superfamily. Angiogenin expression occurs in a variety of tissues with notably pronounced levels in the liver pancreas and placenta indicating its widespread biological importance. In addition to its primary name it appears in the context of research under alternative names like KIT ANG and elisa ang reflecting its use and identification in various experimental assays.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAngiogenin significantly contributes to the process of angiogenesis the formation of new blood vessels from pre-existing vascular structures. It characterizes acts as both a ribonuclease and a potent angiogenic factor. Although not part of a multi-protein complex angiogenin interacts with endothelial cells to promote their growth and migration. Angiogenin facilitates these cellular behaviors by triggering pathways that enhance cell survival and proliferation essential for healthy tissue development and repair.\u003cbr\u003e\nPathways\u003cbr\u003e\nAngiogenin is intricately involved in the angiogenesis pathway a critical process for both physiological and pathological conditions. It interacts with proteins such as VEGF (vascular endothelial growth factor) which plays an essential role in vascular function. Angiogenin's interaction with these pathways enhances cellular responses to external stimuli maintaining vascular integrity and supporting tissue regeneration under stress or injury conditions.\u003cbr\u003e\nAngiogenin has connections to conditions like cancer and amyotrophic lateral sclerosis (ALS). Aberrant regulation of angiogenin can contribute to tumorigenesis by promoting excessive angiogenesis aiding the growth and spread of cancerous cells. In ALS altered angiogenin function associates with neurodegenerative processes linking its function to proteins such as superoxide dismutase 1 (SOD1). Understanding angiogenin's role in these diseases aids in uncovering therapeutic targets and developing treatment strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843630092457,"sku":"ab99970","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab99970","provider":"Iright","version":"1.0","type":"link"}