{"product_id":"biolegend-100206","title":"Biolegend, 100206, PE anti-mouse CD3 Antibody, 200μg","description":"\u003cp\u003eCD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α\/β or γ\/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide\/MHC antigen complex\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: γδTCR-positive T-T hybridoma D1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Zuchtriegel G, et al. 2016. PLoS Biol. 14: 1002459. PubMed Xu C, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016\/j.bbrc.2017.05.071. PubMed Zhao L, et al. 2022. Oncogene. 41:4200. PubMed Guo D, et al. 2023. Cell Biosci. 13:51. PubMed Fan NW, et al. 2023. FASEB J. 37:e22855. PubMed Zhang X, et al. 2023. Front Immunol. 14:1116749. PubMed Sun JY, et al. 2023. Sci Adv. 9:eade4110. PubMed Shi Y, et al. 2023. Nat Commun. 14:1884. PubMed Fiedler T, et al. 2023. J Neuroinflammation. 20:100. PubMed Li Y, et al. 2023. Front Cell Infect Microbiol. 13:1117230. PubMed Bhaskar A, et al. 2023. iScience. 26:106644. PubMed Wang Y, et al. 2023. iScience. 26:106630. PubMed Milighetti M, et al. 2023. iScience. 26:106937. PubMed Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed Hiyoshi H, et al. 2022. Cell Host Microbe. 30:163. PubMed Chan WY, et al. 2022. Infect Immun. 90:e0084618a. PubMed Wang D, et al. 2022. Cell Rep. 39:110587. PubMed Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Yang K, et al. 2022. Sci Immunol. 7:eabn2888. PubMed McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed TaSli PN, et al. 2022. J Food Biochem. 46:e14393. PubMed Shen Z, et al. 2022. EBioMedicine. 85:104285. PubMed Deng P, et al. 2022. J Clin Invest. 132: . PubMed Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed Giannou AD, et al. 2023. Immunity. 56:125. PubMed Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed Correa S, et al. 2020. ACS Nano. 14:2224. PubMed Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed Reboldi A, et al. 2016. Sci Transl Med. 352: aaf4822. PubMed Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed Shen S, et al. 2018. Pain Med. 19:686. PubMed Ushijima M, et al. 2018. Front Immunol. 9:243. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Li Q, et al. 2022. Cell Rep. 40:111308. PubMed Jong RM, et al. 2022. J Immunol. 208:407. PubMed Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed Bedi B, et al. 2012. Proc Natl Acad Sci U S A. 109:275. PubMed Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed Jazowiecka-Rakus J, et al. 2021. Cancers (Basel). 13:. PubMed Schmidt D, et al. 2016. PLoS One. 11: 0159059. PubMed He C, et al. 2020. Clin Transl Med. 10:e39. PubMed Ma C, et al. 2018. Science. 360:eaan5931. PubMed Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed Kuang Z, et al. 2020. Antib Ther. 3:227. PubMed Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed Mathur S, et al. 2021. JCI Insight. 6:. PubMed Ohyagi M, et al. 2021. Nat Commun. 12:7344. PubMed Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed Helm M, et al. 2022. Life (Basel). 12:. PubMed Tu X, et al. 2022. Nat Commun. 13:6977. PubMed Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed Biram A, et al. 2020. Cell Rep. 30:1910. PubMed Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed Mariani SA, et al. 2019. Immunity. 50:1439. PubMed Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed Wu J, et al. 2020. Immunity. 53:115. PubMed Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed Lin H, et al. 2019. Infect Immun. 87:e00055-19. PubMed Pedraza–Zamora CP, et al. 2017. Parasite Immunol. 39:4. PubMed McAlpine CS, et al. 2021. Nature. 595:701. PubMed Wu L, et al. 2022. Theranostics. 12:842. PubMed Goldman E, et al. 2021. Cells. 10:. PubMed Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed Chen Y, et al. 2015. PLoS One. 10: 0135217. PubMed Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed Deng M, et al. 2020. Nat Commun. 11:2193. PubMed Paul S, et al. 2018. Appl Microbiol Biotechnol. 102:8895. PubMed Marinescu CI, et al. 2021. Stem Cell Res Ther. 12:319. PubMed Shi R, et al. 2022. Theranostics. 12:875. PubMed Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed Glaros V, et al. 2021. Immunity. 54:2005. PubMed Hu J, et al. 2022. J Neuroinflammation. 19:71. PubMed O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed Chauveau A, et al. 2020. Immunity. 52:794. PubMed Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed Kim JH, et al. 2019. Mol Med. 25:33. PubMed Pedraza-Zamora C, et al. 2017. Parasite Immunol. 10.1111\/pim.12420. PubMed Fang E, et al. 2022. MedComm (2020). 3:e117. PubMed Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed Lewandowski JP, et al. 2020. Genome Biol. 1.039583333. PubMed Kruta M, et al. 2021. Cell Stem Cell. :. PubMed Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed Huang Z, et al. 2021. Nat Commun. 12:145. PubMed Cho K, et al. 2021. iScience. 24:103117. PubMed Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed Bullard BL, et al. 2022. NPJ Vaccines. 7:65. PubMed Tomala J, et al. 2020. Methods Mol Biol. 2111:101. PubMed Nikolos F, et al. 2022. Nat Commun. 13:1487. PubMed Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed Zou D, et al. 2020. EBioMedicine. 52:102652. PubMed Sanmarco LM, et al. 2021. Nature. 590:473. PubMed Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed Sun J et al. 2018. Cell stem cell. 23(3):355-369 . PubMed Huang L, et al. 2018. Exp Neurol. 300:41. PubMed Ding Y, et al. 2020. Evid Based Complement Alternat Med. 2020:9521073. PubMed Zhang T, et al. 2020. Cell. 180(6):1115-1129. PubMed Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed Feng Y, et al. 2022. J Nanobiotechnology. 20:193. PubMed Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed Yamamura K, et al. 2017. Nat Commun. 8:13946. PubMed Hsieh Y, et al. 2014. Infect Immun . 82:2087. PubMed Winchell CG, et al. 2020. Front Immunol. 1.077083333. PubMed Chan WY, et al. 2019. Infect Immun. 87:. PubMed Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed Schneider A, et al. 2014. J Leukoc Biol. 96:857. PubMed Yao C, et al. 2018. Autophagy. 1.854861111. PubMed Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed Jazowiecka-Rakus J, et al. 2020. Mol Ther Oncolytics. 18:335. PubMed Chen H, et al. 2022. Front Pharmacol. 13:827520. PubMed Zhou T, et al. 2021. Bioact Mater. 0.734027778. PubMed Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed Lu C, et al. 2020. Cancer Cell. 39(1):96-108.e6. PubMed Uluçkan ö, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed Maulhardt HA, et al. 2020. Invest New Drugs. 1618:38. PubMed Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed Fathman J, et al. 2010. Proc Natl Acad Sci U S A. 107:3663. PubMed Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed Sun Y, et al. 2022. iScience. 25:104846. PubMed Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed Wu L, et al. 2020. Cancer Immunol Res. 710:8. PubMed Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Zeng F, et al. 2021. J Invest Dermatol. 596:141. PubMed Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed Song X, et al. 2022. Transl Oncol. 15:101306. PubMed Zuo S, et al. 2021. EBioMedicine. 64:103240. PubMed\u003cbr\u003e\nRRID: AB_312662 (BioLegend Cat. No. 100205) AB_312663 (BioLegend Cat. No. 100206)\u003cbr\u003e\nStructure: Ig superfamily, CD3\/TCR, 20 kD\u003cbr\u003e\nDistribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells\u003cbr\u003e\nFunction: Antigen recognition, TCR signal transduction, T cell activation\u003cbr\u003e\nLigand\/Receptor: Peptide antigen\/MHC-complex\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.\u003cbr\u003e\nGene ID: 12502\u003cbr\u003e\nUniProt: View information about CD3 on UniProt.org\u003cbr\u003e\nClone: 17A2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell antigen receptor complex, T3\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860822577321,"sku":"100206","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-100206","provider":"Iright","version":"1.0","type":"link"}