{"product_id":"biolegend-100619","title":"Biolegend, 100619, Purified anti-mouse CD5 (Maxpar® Ready) Antibody, 100μg","description":"\u003cp\u003eCD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α\/β T cells express high levels of CD5, very few γ\/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1.0 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested CyTOF® - Verified\u003cbr\u003e\nRecommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.\u003cbr\u003e\nAdditional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC) Bourdeau A, et al. 2007. Blood doi:10.1182\/blood-2006-08-044370.\u003cbr\u003e\nRRID: AB_2562763 (BioLegend Cat. No. 100619)\u003cbr\u003e\nStructure: Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD\u003cbr\u003e\nDistribution: Thymocytes, T cells, B-1 cells\u003cbr\u003e\nFunction: Negative regulator of T-B cell interaction\u003cbr\u003e\nLigand\/Receptor: CD72, gp35-37\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.\u003cbr\u003e\nGene ID: 12507\u003cbr\u003e\nUniProt: View information about CD5 on UniProt.org\u003cbr\u003e\nClone: 53-7.3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lyt-1, Ly-1, T1, Tp67, Ly-12\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?\u003cbr\u003e\nA: We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.\u003c\/p\u003e\n\n\u003cp\u003ehttp:\/\/techsupport.fluidigm.com\/\u003cbr\u003e\nQ: Can I use Maxpar® Ready format clones for flow cytometry staining?\u003cbr\u003e\nA: We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.\u003cbr\u003e\nQ: I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.\u003cbr\u003e\nA: We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http:\/\/techsupport.fluidigm.com\/\u003cbr\u003e\nQ: Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?\u003cbr\u003e\nA: The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862441709737,"sku":"100619","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-100619","provider":"Iright","version":"1.0","type":"link"}