{"product_id":"biolegend-100626","title":"Biolegend, 100626, APC anti-mouse CD5 Antibody, 100μg","description":"\u003cp\u003eCD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α\/β T cells express high levels of CD5, very few γ\/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC) Bourdeau A, et al. 2007. Blood doi:10.1182\/blood-2006-08-044370.\u003cbr\u003e\nProduct Citations: Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed Worth AN, et al. 2022. Cell Rep. 39:110899. PubMed Jennings EK, et al. 2021. STAR Protocols. 2(1):100284. PubMed Han J, et al. 2018. BMC Res Notes. 11:229. PubMed Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed Kruta M, et al. 2021. Cell Stem Cell. :. PubMed Kim S, et al. 2021. Elife. 10:. PubMed Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Dong X, et al. 2022. Front Immunol. 13:896472. PubMed Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed Werner A, et al. 2021. iScience. 24:103076. PubMed Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed Corrado M, et al. 2020. Cell Metab. 32:981. PubMed Goc J, et al. 2021. Cell. 184:5015. PubMed Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed Liang Z, et al. 2022. iScience. 25:105233. PubMed\u003cbr\u003e\nRRID: AB_2563928 (BioLegend Cat. No. 100625) AB_2563929 (BioLegend Cat. No. 100626)\u003cbr\u003e\nStructure: Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD\u003cbr\u003e\nDistribution: Thymocytes, T cells, B-1 cells\u003cbr\u003e\nFunction: Negative regulator of T-B cell interaction\u003cbr\u003e\nLigand\/Receptor: CD72, gp35-37\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.\u003cbr\u003e\nGene ID: 12507\u003cbr\u003e\nUniProt: View information about CD5 on UniProt.org\u003cbr\u003e\nClone: 53-7.3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lyt-1, Ly-1, T1, Tp67, Ly-12\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862440726697,"sku":"100626","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-100626","provider":"Iright","version":"1.0","type":"link"}