{"product_id":"biolegend-100710","title":"Biolegend, 100710, PE\/Cyanine5 anti-mouse CD8a Antibody, 100μg","description":"\u003cp\u003eCD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)\/β(CD8b) heterodimer and α\/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α\/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α\/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α\/α homodimer is expressed on a subset of γ\/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE\/Cyanine5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.04 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP) Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete) Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP) Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block) Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC) Shih FF, et al. 2006. J. Immunol. 176:3438. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete) Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed Lee CH, et al. 2009. Clin. Cancer Res. PubMed Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed Guo Y, et al. 2008. Blood 112:480. PubMed Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Medyouf H, et al. 2010. Blood 115:1175. PubMed Riedl P, et al. 2009. J. Immunol. 183:370. PubMed Apte SH, et al. 2010. J. Immunol. 185:998. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Cui L, et al. 2015. J Control Release. 206:220. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed Wang Z, et al. 2023. J Cancer. 14:1049. PubMed Erickson JJ, et al. 2022. Nature. 606:769. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed Tan L, et al. 2022. Aging Dis. 13:1562. PubMed Hofmann I, et al. 2022. J Pharmacol Exp Ther. Online ahead of print. PubMed Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed Kurosawa M, et al. 2021. Int J Mol Sci. 22:. PubMed Karagiannidis I, et al. 2020. Front Immunol. 11:1885. PubMed Stolp B, et al. 2022. Cell Rep. 38:110387. PubMed Ihara S, et al. 2022. PLoS Negl Trop Dis. 16:e0010323. PubMed Sehnert B, et al. 2022. Front Immunol. 13:921488. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed Sharma PK, et al. 2018. Cancer Gene Ther. 25:27. PubMed Pein M, et al. 2020. Nat Commun. 11:1494. PubMed Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed Gungor B, et al. 2014. Sci Transl Med. 6:235. PubMed Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed Cervera–Carrascon V, et al. 2020. Oncoimmunology. 9:1761229. PubMed Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed Wachsmuth LP, et al. 2020. Biol Blood Marrow Transplant. 26:230. PubMed Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed Yankova E, et al. 2021. Nature. 593:597. PubMed Ma P, et al. 2014. Diabetes. 63:3458. PubMed Luck H, et al. 2015. Cell Metab. 21 527 . PubMed Sim S, et al. 2016. PLoS One. 11: 0152485. PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed XS R, et al. 2015. Diabetes. 64 90. PubMed Naik A, et al. 2015. J Immunol. 194:790. PubMed Tomay F, et al. 2019. J Transl Med. 17:237. PubMed Yang R, et al. 2022. J Adv Res. 35:259. PubMed Todd D, et al. 2009. J Exp Med. 206:2151. PubMed Edwards-Hicks J, et al. 2020. Metabolites. 11:. PubMed Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed Luck H, et al. 2019. Nat Commun. 10:3650. PubMed Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed Zhang Q, et al. 2022. Commun Biol. 5:544. PubMed Lo W, et al. 2014. Elife. 14:1457. PubMed Bryan Hart, Sunhee Lee 2016. PLoS Negl Trop Dis. 10:e0005229. PubMed Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed Zhou S, et al. 2021. Adv Funct Mater. 31:. PubMed Hart B, et al. 2015. PLoS Negl Trop Dis. 9: 0004046. PubMed Liang Z, et al. 2017. Autophagy. 14:505. PubMed Chavez JS, et al. 2022. Cells. 11:. PubMed Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed Tsai S, et al. 2018. Cell Metab. 28:922. PubMed\u003cbr\u003e\nRRID: AB_312748 (BioLegend Cat. No. 100709) AB_312749 (BioLegend Cat. No. 100710)\u003cbr\u003e\nStructure: Ig superfamily, CD8α chain, 34 kD\u003cbr\u003e\nDistribution: Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells\u003cbr\u003e\nFunction: Co-receptor for TCR\u003cbr\u003e\nLigand\/Receptor: MHC class I molecule\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Zamoyska R. 1994. Immunity 1:243. 3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.\u003cbr\u003e\nGene ID: 12525\u003cbr\u003e\nUniProt: View information about CD8alpha on UniProt.org\u003cbr\u003e\nClone: 53-6.7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T8, Lyt2, Ly-2\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862433484969,"sku":"100710","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-100710","provider":"Iright","version":"1.0","type":"link"}