{"product_id":"biolegend-101308","title":"Biolegend, 101308, PE anti-mouse CD16\/32 Antibody, 200μg","description":"\u003cp\u003eCD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16\/CD32 are expressed on B cells, monocytes\/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Sorted pre-B cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone 93 can be used for blocking of CD16\/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.The 93 mAb is specific to the common epitope of CD16\/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16\/CD32 antibody (=1.0 µg per 106 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin \u0026lt;0.01 EU\/µg, Azide-Free, 0.2 µm filtered).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Personal communication (IP) Oliver AM, et al. 1999. Hybridoma 18:113. (Block) Brummel R and Lenert P. 2005. J. Immunol. 174:2429. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block) Clements JL, et al. 2006. J. Immunol. 177:905. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed Kmieciak M, et al. 2011. J. Vis. Exp. 47:2381. PubMed Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed Koon HW, et al. 2013. J. Vis. Exp. 68:4208. PubMed Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed Huang J, et al. 2013. J. Immunol Methods. 387:254. PubMed Dutow P, et al. 2014. J Infect Dis. PubMed Fan Y, et al. 2014. J Exp Med. 211:313. PubMed Huang HN, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed Takei S, et al. 2014. Vaccine. 32:3066. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed Kimura T, et al. 2014. Int Immunol. 26:697. PubMed Everad A, et al. 2014. Nat Commun. 5:5648. PubMed Cenci E, et al. 2006. J. Leuko. Biol. 79(1):40-5. (Block)\u003cbr\u003e\nProduct Citations: Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed Pannunzio B, et al. 2022. Cells. 11: . PubMed Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed Ye B, et al. 2022. Cell Mol Immunol. 19:619. PubMed Davis FM, et al. 2020. J Immunol. 2503:204. PubMed Domingues AF, et al. 2020. Elife. 9:e51754. PubMed Li DY, et al. 2018. Sci Rep. 8:17979. PubMed Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed Hanhai Z, et al. 2021. Aging (Albany NY). 13:13108. PubMed Chen L, et al. 2022. J Nanobiotechnology. 20:210. PubMed Xiao S, et al. 2008. J Immunol. 180:914. PubMed Sun Y et al. 2018. Cancer cell. 34(4):643-658 . PubMed Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390\/ijms18051037. PubMed Amo-Aparicio J, et al. 2021. Theranostics. 11:9805. PubMed Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed Dong S, et al. 2021. Nature. 591:117. PubMed Martìnez-Muriana A, et al. 2016. Sci Rep. 6: 25663. PubMed Zaro BW, et al. 2020. eLife. 9:00. PubMed Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed Zegarra‐Ruiz DF et al. 2018. Cell host \u0026amp; microbe. 25(1):113-127 . PubMed Kojima K, et al. 2011. Am J Respir Crit Care Med. 184:328. PubMed Rai S, et al. 2022. Nat Commun. 13:5346. PubMed Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed Nakagawa MM, et al. 2018. Cell Rep. 25:2094. PubMed Li J, et al. 2013. J Immunol. 190:597. PubMed Wünsche P et al. 2018. Cell stem cell. 23(1):132-146 . PubMed Giotopoulos G, et al. 2015. J Exp Med. 212: 1551-1569. PubMed Hohsfield LA, et al. 2021. Elife. 10:. PubMed\u003cbr\u003e\nRRID: AB_312806 (BioLegend Cat. No. 101307) AB_312807 (BioLegend Cat. No. 101308)\u003cbr\u003e\nStructure: Ig superfamily, 40-60 kD\u003cbr\u003e\nDistribution: B cells, monocyte\/macrophages, NK cells, neutrophils, mast cells, dendritic cells\u003cbr\u003e\nFunction: Low affinity receptors for IgG\u003cbr\u003e\nLigand\/Receptor: IgG\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Unkeless JC. 1989. J. Clin. Invest. 83:355. 3. Qiu WQ, et al. 1990. Science 248:732.\u003cbr\u003e\nGene ID: 1413014131\u003cbr\u003e\nUniProt: View information about CD16\/32 on UniProt.org\u003cbr\u003e\nClone: 93\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Fcγ R III\/II, Ly-17\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862501314729,"sku":"101308","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-101308","provider":"Iright","version":"1.0","type":"link"}