{"product_id":"biolegend-101608","title":"Biolegend, 101608, PE anti-mouse CD23 Antibody, 200μg","description":"\u003cp\u003eCD23 is a 45 kD protein also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, immunohistochemical staining of acetone-fixed frozen sections5, and spatial biology (IBEX)7,8.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP) Rao M, et al. 1987. J. Immunol. 138:1845. (Block) Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block) Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block) Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC) Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed Chiu H, et al. 2021. iScience. 24:102748. PubMed Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed Wu J, et al. 2022. Nat Commun. 13:7321. PubMed Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed Uchil PD et al. 2018. Cell host \u0026amp; microbe. 25(1):87-100 . PubMed Lee-Chang C, et al. 2016. J Immunol. 196: 3385-3397. PubMed McGuire A, et al. 2016. Nat Commun. 7:10618. PubMed Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed Chen F et al. 2018. Cell reports. 25(10):2775-2783 . PubMed Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed Zhao X, et al. 2017. Nat Med. 10.1038\/nm.4260. PubMed Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed Aschman T, et al. 2021. Int J Mol Sci. 22:. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Wang D, et al. 2005. Int Immunol. 1.527083333. PubMed Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed Lu X, et al. 2019. Cell Rep. 28:472. PubMed Escolano A, et al. 2016. Cell. 166(6):1445-1458.e12. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed Haines RR, et al. 2019. J Immunol. 203:1867. PubMed Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed\u003cbr\u003e\nRRID: AB_312832 (BioLegend Cat. No. 101607) AB_312833 (BioLegend Cat. No. 101608)\u003cbr\u003e\nStructure: C-lectin family, type II transmembrane glycoprotein, 45 kD\u003cbr\u003e\nDistribution: B cells, follicular dendritic cells\u003cbr\u003e\nFunction: Regulates B cell activation\u003cbr\u003e\nLigand\/Receptor: IgE, CD19\/CD21\/CD81\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Delespesse G, et al. 1992. Immunol. Rev. 125:77. 3. Flores-Romo L, et al. 1993. Science 261:1038.\u003cbr\u003e\nGene ID: 14128\u003cbr\u003e\nUniProt: View information about CD23 on UniProt.org\u003cbr\u003e\nClone: B3B4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862891778217,"sku":"101608","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-101608","provider":"Iright","version":"1.0","type":"link"}