{"product_id":"biolegend-102415","title":"Biolegend, 102415, Alexa Fluor® 647 anti-mouse CD31 Antibody, 25μg","description":"\u003cp\u003eCD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α v β 3 integrin, CD51\/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: C3H\/HeJ mouse hematopoietic progenitor cell line 3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality testedIHC-F - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm \/ 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Baldwin HS, et al. 1994. Development 120:2539. (IP, Block) DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block) Rosenblum WI, et al. 1996. Stroke 27:709. (Block) Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block) Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC) Wiewrodt R, et al. 2002. Blood 99:912. (IHC) McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed Chacko AM, et al. 2012. PLoS One 7:e34958. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed\u003cbr\u003e\nProduct Citations: Zhu J, et al. 2023. Cell Rep Methods. 3:100407. PubMed Wan P, et al. 2021. Neurophotonics. 8:035007. PubMed Liu L, et al. 2022. Elife. 11: . PubMed Zhu J, et al. 2022. Neurophotonics. 9:045008. PubMed Lambert AW, et al. 2022. Dev Cell. 57:2714. PubMed Li Y, et al. 2023. Theranostics. 13:403. PubMed Miyawaki T, et al. 2020. Nat Commun. 1.225. PubMed Soveg F, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L573-L583. PubMed McQualter J, et al. 2009. Stem Cells. 27:623. PubMed Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed Berdnikovs S, et al. 2013. Am J Physiol Lung Cell Mol Physiol. 304:240. PubMed Zhu J, et al. 2020. Adv Sci (Weinh). 7:1903185. PubMed Plant T, et al. 2020. J Clin Invest. 130:3221. PubMed Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed Qi Y, et al. 2019. Sci Adv. 5:eaau8355. PubMed Graney PL, et al. 2020. Sci Adv. 6:eaay6391. PubMed Akram KM, et al. 2019. Nat Commun. 10:1178. PubMed Matryba P, et al. 2020. J Biophotonics. 13:e202000072. PubMed Jagannathan P, et al. 2017. Sci Rep. 10.1038\/s41598-017-10624-3. PubMed Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed De Niz M, et al. 2021. Cell Rep. 36:109741. PubMed Roncato F, et al. 2021. Cell Adhesion Migration. 15(1):166-179. PubMed Michael BD, et al. 2020. Cell Reports. 32(11):108150. PubMed Moretti FA, et al. 2018. Elife. 7:e35816. PubMed Zotter B, et al. 2022. J Neurosci. 42:183. PubMed Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed Shanahan MT, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 321:G668. PubMed Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed Matryba P, et al. 2020. J Immunol. 1395:204. PubMed Esposito M, et al. 2019. Nat Cell Biol. 21:627. PubMed Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed Mori Y, et al. 2014. Sci Rep. 4:6997. PubMed Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed Park JK, et al. 2018. Stem Cell Res Ther. 9:197. PubMed Dudeck J, et al. 2021. Immunity. 54(3):468-483.e5. PubMed Stalin J, et al. 2016. Oncogene. 10.1038\/onc.2016.83. PubMed Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed Lui BG, et al. 2020. Nat Commun. 0.663194444. PubMed Silva HM, et al. 2019. J Exp Med. 216:786. PubMed Riedel S, et al. 2012. PLoS One. 7:e52398. PubMed Chojnacki A, et al. 2019. Commun Biol. 2:181. PubMed Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed De Niz M, et al. 2022. STAR Protoc. 3:101450. PubMed Oni TE, et al. 2020. J Exp Med. :217. PubMed Dougan M, et al. 2018. Cancer Immunol Res. 6:389. PubMed\u003cbr\u003e\nRRID: AB_493411 (BioLegend Cat. No. 102415) AB_493410 (BioLegend Cat. No. 102416)\u003cbr\u003e\nStructure: Ig superfamily, 130-140 kD\u003cbr\u003e\nDistribution: Endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, T and B cell subsets\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: CD38, αV\/β3 integrin\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.\u003cbr\u003e\nGene ID: 18613\u003cbr\u003e\nUniProt: View information about CD31 on UniProt.org\u003cbr\u003e\nClone: 390\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PECAM-1, EndoCAM\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862383022249,"sku":"102415","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-102415","provider":"Iright","version":"1.0","type":"link"}