{"product_id":"biolegend-102446","title":"Biolegend, 102446, Ultra-LEAF™ Purified anti-mouse CD31 Antibody, 1mg","description":"\u003cp\u003eCD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α v β 3 integrin, CD51\/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.\u003cbr\u003e\n1mg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: C3H\/HeJ mouse hematopoietic progenitor cell line 3\u003cbr\u003e\nFormulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.\u003cbr\u003e\nEndotoxin Level: Less than 0.01 EU\/µg of the protein (\u0026lt; 0.001 ng\/µg of the protein) as determined by the LAL test.\u003cbr\u003e\nPreparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL. Older lots may have also been bottled at 1 mg\/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Baldwin HS, et al. 1994. Development 120:2539. (IP, Block) DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block) Rosenblum WI, et al. 1996. Stroke 27:709. (Block) Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block) Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC) Wiewrodt R, et al. 2002. Blood 99:912. (IHC) McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed Chacko AM, et al. 2012. PLoS One 7:e34958. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed\u003cbr\u003e\nRRID: AB_2832290 (BioLegend Cat. No. 102445) AB_2832291 (BioLegend Cat. No. 102446)\u003cbr\u003e\nStructure: Ig superfamily, 130-140 kD\u003cbr\u003e\nDistribution: Endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, T and B cell subsets\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: CD38, αV\/β3 integrin\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.\u003cbr\u003e\nGene ID: 18613\u003cbr\u003e\nUniProt: View information about CD31 on UniProt.org\u003cbr\u003e\nClone: 390\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PECAM-1, EndoCAM\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: Do you guarantee that your antibodies are totally pathogen free?\u003cbr\u003e\nA: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?\u003cbr\u003e\nA: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?\u003cbr\u003e\nA: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.\u003cbr\u003e\nQ: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?\u003cbr\u003e\nA: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862384136361,"sku":"102446","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-102446","provider":"Iright","version":"1.0","type":"link"}