{"product_id":"biolegend-105316","title":"Biolegend, 105316, Alexa Fluor® 488 anti-mouse CD90.2 (Thy1.2) Antibody, 100μg","description":"\u003cp\u003eCD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb\/c, CBA\/J, C3H\/He, C57BL\/-, DBA, NZB\/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca 2+ flux in thymocytes and, in combination with antibody against the CD3\/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, a concentration range of 5.0 - 10 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3\/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR\/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16\/CD32, clone 93 (Cat. No. 101301 \u0026amp; 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt;0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 \u0026amp; 105352).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete) Seaman WE. 1983. J. Immunol. 130:1713. (Deplete) Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim) Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC) Hardy B, et al. 2005. Int. Immunol. 17:615. Drobyski W, et al. 1996. Blood 87:5355. (Deplete) Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed Sungur CM, et al. 2013. PNAS. 110:7401. PubMed\u003cbr\u003e\nProduct Citations: Zheng P, et al. 2023. Stroke Vasc Neurol. . PubMed Yeh CH, et al. 2022. Immunity. 55:272. PubMed Todd E, et al. 2011. J Immunol. 187:3015. PubMed Wu N, et al. 2020. Cell Rep. 30:1129. PubMed Amano C, et al. 2015. PLoS One. 10: 0137207. PubMed Kretschmer S, et al. 2013. PLoS One. 8:e55201. PubMed Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed Armaka M, et al. 2022. Genome Med. 14:78. PubMed García-Bonilla M, et al. 2020. Stem Cell Res Ther. 11:121. PubMed Schliehe C, et al. 2012. J Virol. 139:3442. PubMed Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed Armaka M, et al. 2018. Nat Commun. 9:618. PubMed Mohammed RN, et al. 2019. Sci Rep. 4.185416667. PubMed Prasai A, et al. 2017. Stem Cell Rev.. 10.1007\/s12015-017-9721-9. PubMed\u003cbr\u003e\nRRID: AB_492887 (BioLegend Cat. No. 105315) AB_492886 (BioLegend Cat. No. 105316)\u003cbr\u003e\nStructure: Ig superfamily, 25-35 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice\u003cbr\u003e\nFunction: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells\u003cbr\u003e\nLigand\/Receptor: CD45\u003cbr\u003e\nCell Type: Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Craig W, et al. 1993. J. Exp. Med. 177:1331. 3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1. 4. Mayani H, et al. 1994. Blood 83:2410.\u003cbr\u003e\nGene ID: 21838\u003cbr\u003e\nUniProt: View information about CD90.2 on UniProt.org\u003cbr\u003e\nClone: 30-H12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Thy-1.2\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862364868777,"sku":"105316","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-105316","provider":"Iright","version":"1.0","type":"link"}