{"product_id":"biolegend-105322","title":"Biolegend, 105322, PerCP anti-mouse CD90.2 (Thy1.2) Antibody, 100μg","description":"\u003cp\u003eCD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb\/c, CBA\/J, C3H\/He, C57BL\/-, DBA, NZB\/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca 2+ flux in thymocytes and, in combination with antibody against the CD3\/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The CD90.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3\/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR\/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16\/CD32, clone 93 (Cat. No. 101301 \u0026amp; 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt;0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 \u0026amp; 105352).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete) Seaman WE. 1983. J. Immunol. 130:1713. (Deplete) Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim) Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC) Hardy B, et al. 2005. Int. Immunol. 17:615. Drobyski W, et al. 1996. Blood 87:5355. (Deplete) Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed Sungur CM, et al. 2013. PNAS. 110:7401. PubMed\u003cbr\u003e\nProduct Citations: Quispe Calla N, et al. 2016. Sci Rep. 6:37723. PubMed Price J, et al. 2015. Diabetes. 64: 3521 - 3531. PubMed Emgård J, et al. 2018. Immunity. 143:419. PubMed Thompson E, et al. 2010. J Exp Med. 207:1791. PubMed Knocke S, et al. 2016. Cell Rep. 17:2234-2246. PubMed Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed Lai NY, et al. 2020. Cell. 180:33:00. PubMed Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed Bachy E, et al. 2016. J Exp Med. 213: 841 - 857. PubMed\u003cbr\u003e\nRRID: AB_893454 (BioLegend Cat. No. 105321) AB_893453 (BioLegend Cat. No. 105322)\u003cbr\u003e\nStructure: Ig superfamily, 25-35 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice\u003cbr\u003e\nFunction: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells\u003cbr\u003e\nLigand\/Receptor: CD45\u003cbr\u003e\nCell Type: Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Craig W, et al. 1993. J. Exp. Med. 177:1331. 3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1. 4. Mayani H, et al. 1994. Blood 83:2410.\u003cbr\u003e\nGene ID: 21838\u003cbr\u003e\nUniProt: View information about CD90.2 on UniProt.org\u003cbr\u003e\nClone: 30-H12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Thy-1.2\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862365032617,"sku":"105322","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-105322","provider":"Iright","version":"1.0","type":"link"}