{"product_id":"biolegend-105701","title":"Biolegend, 105701, Purified anti-mouse CD106 Antibody, 50μg","description":"\u003cp\u003eCD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α 4 \/β 1 integrin) and LPAM-1 (α 4 \/β 7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse preadipose cell line PA6\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality testedIHC-F, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, immunoprecipitation1 , and spacial biology (IBEX)11,12. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 \u0026amp; 105728).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC) Kinashi T, et al. 1994. Blood Cells 20:25. (Block) Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC) Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC) Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed Monnier J, et al. 2012. J. Immunol. 189:956. PubMed Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Kimura T, et al. 2021. Development. 148: . PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Dertschnig S, et al. 2020. J Clin Invest. 130:1896. PubMed Tedford K, et al. 2017. Nat Commun. . 10.1038\/s41467-017-02482-4. PubMed Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed Lorsung RM, et al. 2020. Front Bioeng Biotechnol. 0.9375. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Wang C, et al. 2014. J Exp Med. 211:395. PubMed An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed\u003cbr\u003e\nRRID: AB_313202 (BioLegend Cat. No. 105701) AB_313203 (BioLegend Cat. No. 105702)\u003cbr\u003e\nStructure: Ig superfamily, 47 kD\u003cbr\u003e\nDistribution: Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: VLA-4 (α4\/β1 integrin) and LPAM-1 (α4\/β7 integrin)\u003cbr\u003e\nCell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. 3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767. 4. Koni PA, et al. 2001. J. Exp. Med. 193:741.\u003cbr\u003e\nGene ID: 22329\u003cbr\u003e\nUniProt: View information about CD106 on UniProt.org\u003cbr\u003e\nClone: 429 (MVCAM.A)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: VCAM-1, INCAM-110\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862403174569,"sku":"105701","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-105701","provider":"Iright","version":"1.0","type":"link"}