{"product_id":"biolegend-105802","title":"Biolegend, 105802, Purified anti-mouse CD117 (c-Kit) Antibody, 500μg","description":"\u003cp\u003eCD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and\/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse bone marrow mast cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality testedIHC-F - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC) Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC) Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC) Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Tarazi S, et al. 2022. Cell. 185:3290. PubMed Li F, et al. 2020. EMBO J. 39:e103205. PubMed Van Deren DA, et al. 2022. Development. 149: . PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed Podd B, et al. 2006. J Immunol. 176:6532. PubMed Sereni L, et al. 2018. J Allergy Clin Immunol. 142:1272. PubMed Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed Liu Y, et al. 2021. Nat Commun. 12:6323. PubMed Martin K,et al. 2017. J Immunol.. 10.4049\/jimmunol.1601522. PubMed Batsivari A, et al. 2017. Stem Cell Reports. 8:1549. PubMed Zhong X, et al. 2020. Proc Natl Acad Sci U S A. 8563:117. PubMed Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed Wicki S, et al. 2018. Int J Mol Sci. 1.266666667. PubMed Matsuba S, et al. 2017. Front Immunol. . 10.3389\/fimmu.2017.01538. PubMed Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed Morita S et al. 2017. Cell metabolism. 25(4):883-897 . PubMed Schuler CF, et al. 2020. Allergy. 75:2279. PubMed Hsieh HY, et al. 2020. Cancer Sci. 111:2400. PubMed Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed Verbiest T, et al. 2018. Leukemia. 32:1435. PubMed Bachelet I, et al. 2008. J Immunol. 180:6064. PubMed Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed Russell AL, et al. 2021. Mol Ther Methods Clin Dev. 21:710. PubMed Lin Z, et al. 2021. Stem Cells. 39:240. PubMed Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed Coelho P, et al. 2015. Open Bio. 5: 150209. PubMed Gu Z, et al. 2019. Cancer Discov. 1.227777778. PubMed\u003cbr\u003e\nRRID: AB_313210 (BioLegend Cat. No. 105801) AB_313211 (BioLegend Cat. No. 105802)\u003cbr\u003e\nStructure: Ig superfamily, 145 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells, AML, mast cells\u003cbr\u003e\nFunction: Growth factor receptor, tyrosine kinase\u003cbr\u003e\nLigand\/Receptor: Stem Cell Factor (SCF)\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5.\u003cbr\u003e\nGene ID: 16590\u003cbr\u003e\nUniProt: View information about CD117 on UniProt.org\u003cbr\u003e\nClone: 2B8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: c-KIT, Stem Cell Factor Receptor (SCFR)\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866541707433,"sku":"105802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-105802","provider":"Iright","version":"1.0","type":"link"}