{"product_id":"biolegend-105808","title":"Biolegend, 105808, PE anti-mouse CD117 (c-Kit) Antibody, 200μg","description":"\u003cp\u003eCD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and\/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse bone marrow mast cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC) Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC) Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC) Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed Cacialli P, et al. 2022. EMBO J. 41:e108536. PubMed Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed Boers R, et al. 2023. Nat Biotechnol. . PubMed Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed Capellmann S, et al. 2023. Front Immunol. 14:1154416. PubMed van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed Casellas Roman HL, et al. 2020. Leuk Res. 94:106372. PubMed Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed Kropp L, et al. 2020. Clin Exp Allergy. 50:213. PubMed Cela D, et al. 2022. J Leukoc Biol. 111:1235. PubMed Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Roy IM, et al. 2022. iScience. 25:105171. PubMed Chen Y, et al. 2023. Stem Cells Int. 2023:7482546. PubMed Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed Li X, et al. 2022. Cell. 185:1709. PubMed Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed Kakiuchi K, et al. 2014. Biol Reprod. 90:82. PubMed Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed Nguyen N, et al. 2022. iScience. 25:103679. PubMed Schmitt M et al. 2018. Cell reports. 24(9):2312-2328 . PubMed Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed Tamura M, et al. 2019. Sci Rep. 9:8171. PubMed Svahn S, et al. 2015. Infect Immun . 83:514. PubMed Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed Klimovich B, et al. 2022. J Exp Clin Cancer Res. 41:80. PubMed Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed Nakano N, et al. 2011. J Biol Chem. 286:12042. PubMed Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed Wu L, et al. 2022. Theranostics. 12:842. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Liu T, et al. 2022. Front Immunol. 13:901349. PubMed Cho K, et al. 2021. iScience. 24:103117. PubMed Qian J, et al. 2021. Int J Mol Sci. 22:. PubMed Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed Yang T, et al. 2022. Nat Commun. 13:4464. PubMed Ding P, et al. 2022. Bone Res. 10:42. PubMed Svahn S, et al. 2016. Infect Immun . 84: 1205-1213. PubMed Pervolarakis N, et al. 2020. Cell Rep. 108273:33. PubMed Quick M, et al. 2012. Am J Physiol Regul Integr Comp Physiol. 303:580. PubMed Bennett FC, et al. 2018. Neuron. 98:1170. PubMed Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed Grainger N, et al. 2020. J Physiol. 598:3283. PubMed Shu Z, et al. 2020. Mar Drugs. 18:00. PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed Sackstein C 2014. Proc Natl Acad Sci U S A. 111:10696. PubMed Yamazaki S, et al. 2015. J Immunol. 195: 3427 - 3435. PubMed Asada S, et al. 2018. Nat Commun. 9:2733. PubMed Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed Ghazaryan S, et al. 2014. Mol Cell Biol. 34:2833. PubMed Zhong C, et al. 2020. Immunity. 52(1):83-95.e4.. PubMed Sakashita A, et al. 2019. Biol Reprod. 100:697. PubMed Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed Takayama G, et al. 2013. Int Immunol. 25:215. PubMed Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed Wang C, et al. 2008. Am J Physiol Regul Integr Comp Physiol. 294:R811. PubMed Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed\u003cbr\u003e\nRRID: AB_313216 (BioLegend Cat. No. 105807) AB_313217 (BioLegend Cat. No. 105808)\u003cbr\u003e\nStructure: Ig superfamily, 145 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells, AML, mast cells\u003cbr\u003e\nFunction: Growth factor receptor, tyrosine kinase\u003cbr\u003e\nLigand\/Receptor: Stem Cell Factor (SCF)\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5.\u003cbr\u003e\nGene ID: 16590\u003cbr\u003e\nUniProt: View information about CD117 on UniProt.org\u003cbr\u003e\nClone: 2B8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: c-KIT, Stem Cell Factor Receptor (SCFR)\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866541314217,"sku":"105808","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-105808","provider":"Iright","version":"1.0","type":"link"}