{"product_id":"biolegend-105824","title":"Biolegend, 105824, PerCP\/Cyanine5.5 anti-mouse CD117 (c-kit) Antibody, 100μg","description":"\u003cp\u003eCD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and\/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse bone marrow mast cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC) Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC) Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC) Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Krauss J, et al. 2015. Proc Natl Acad Sci U S A. 112: 10455-10460. PubMed Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed Chen S, et al. 2022. Cancer Discov. :. PubMed De Koninck M, et al. 2020. Cell Reports. 32(6):108014. PubMed Winn H 2010. Toxicol Sci. 113:207. PubMed Inoue D, et al. 2021. Nat Genet. 53:707. PubMed Yoshimi A, et al. 2019. Nature. 574:273. PubMed Meixiong J et al. 2019. Immunity. 50(5):1163-1171 . PubMed Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed Yamada D, et al. 2014. J Immunol. 192:4112. PubMed Chillo O, et al. 2016. Cell Rep. 16:2197-2207. PubMed Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed Ledo JH, et al. 2020. PLoS One. e0237773:15. PubMed Schuler F, et al. 2017. Nat Commun. . 10.1038\/s41467-017-01850-4. PubMed Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed Ma Y, et al. 2009. Blood. 114:4402. PubMed Yamazaki S, et al. 2015. J Immunol. 195: 3427 - 3435. PubMed Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed Zeng F, et al. 2021. J Invest Dermatol. 596:141. PubMed Ledo JH, et al. 2020. Mol Psychiatry. . PubMed\u003cbr\u003e\nRRID: AB_2131598 (BioLegend Cat. No. 105823) AB_2131597 (BioLegend Cat. No. 105824)\u003cbr\u003e\nStructure: Ig superfamily, 145 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells, AML, mast cells\u003cbr\u003e\nFunction: Growth factor receptor, tyrosine kinase\u003cbr\u003e\nLigand\/Receptor: Stem Cell Factor (SCF)\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5.\u003cbr\u003e\nGene ID: 16590\u003cbr\u003e\nUniProt: View information about CD117 on UniProt.org\u003cbr\u003e\nClone: 2B8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: c-KIT, Stem Cell Factor Receptor (SCFR)\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866541641897,"sku":"105824","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-105824","provider":"Iright","version":"1.0","type":"link"}