{"product_id":"biolegend-105912","title":"Biolegend, 105912, APC anti-mouse CD122 (IL-2Rβ) Antibody, 100μg","description":"\u003cp\u003eCD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γ c , CD132) alone or with the γ c subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Rat myeloma YB2\/0 transfected with truncated mouse Il-2Rβ cDNA\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,2.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Furse R, et al. 1993 Eur. J. Immunol. 23:3181. (IP) He YW, et al. 1995. J. Immunol. 154:1596. (IP) Liqons DL, et al. 2012. J Biol Chem. 287:34386. PubMed.\u003cbr\u003e\nProduct Citations: Siepe DH, et al. 2022. Elife. 11:. PubMed\u003cbr\u003e\nRRID: AB_2800565 (BioLegend Cat. No. 105911) AB_2800566 (BioLegend Cat. No. 105912)\u003cbr\u003e\nStructure: Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD\u003cbr\u003e\nDistribution: T cells and B cells, NK cells, monocytes, macrophages\u003cbr\u003e\nFunction: Critical component of IL-2 and IL-15 signaling\u003cbr\u003e\nLigand\/Receptor: IL-2, IL-15\u003cbr\u003e\nCell Type: B cells, Macrophages, Monocytes, NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245. 3. Suzuki H, et al. 1995. Science 268:1472. 4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.\u003cbr\u003e\nGene ID: 16185\u003cbr\u003e\nUniProt: View information about CD122 on UniProt.org\u003cbr\u003e\nClone: 5H4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-2 Receptor β chain, IL-2Rβ\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862463795369,"sku":"105912","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-105912","provider":"Iright","version":"1.0","type":"link"}