{"product_id":"biolegend-108105","title":"Biolegend, 108105, FITC anti-mouse Ly-6A\/E (Sca-1) Antibody, 50μg","description":"\u003cp\u003eLy-6A\/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A\/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA\/2, SJL, SWR, and 129), Ly-6A\/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB\/c, CBA, C3H\/He, DBA\/1, and NZB) have low Ly-6A\/E expression on resting peripheral lymphocytes. The expression of Ly-6A\/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A\/E is thought to be involved in the regulation of both T and B cell responses.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: IL-2-dependent mouse T-cell line (CTL-L)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9. The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP) Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB) Codias EK, et al. 1990. J. Immunol. 144:2197. Malek TR, et al. 1986. J. Exp. Med. 164:709. Codias EK, et al. 1990. J. Immunol. 145:1407. Ivanov V, et al. 1994. J. Immunol. 153:2394. Karlhofer FM, et al. 1991. J. Immunol. 146:3662. Fleming T, et al. 1994. J. Immunol. 153:1955. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC) Umland O, et al. 2007. J. Immunol. 178:4147. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed Pronk CJ, et al. 2011. J. Exp Med. PubMed English A, et al. 2000. J. Immunol. 165:3763. (IHC) Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed\u003cbr\u003e\nProduct Citations: Abdelwahab T, et al. 2023. iScience. 26:106698. PubMed Shao X, et al. 2023. Cell Discov. 9:44. PubMed Zhao M, et al. 2022. Nat Commun. 13:7600. PubMed Lee DE, et al. 2022. Nat Commun. 13:7613. PubMed Ludwik KA, et al. 2020. Cell Reports. 32(3):107931. PubMed Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed Yamaguchi A, et al. 2021. Genes Cells. 26:782. PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Mittal S, et al. 2016. Stem Cell Reports. 7:583-590. PubMed Hughes RM, et al. 2020. Cancer Research. 79(14):3636-3650.. PubMed Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed Cha S, et al. 2021. Int J Mol Sci. 22:. PubMed Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed Wu L, et al. 2022. Theranostics. 12:842. PubMed Lima-Junior DS, et al. 2021. Cell. . PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed Kim JH, et al. 2021. J Cachexia Sarcopenia Muscle. 12:177. PubMed Kim J, et al. 2016. Nat Cell Biol. 18:930-940. PubMed Zheng X, et al. 2015. Cell Rep. 13:1505-1518. PubMed Kanemaru K, et al. 2015. PLoS One. 10: 0144436. PubMed Ghosh D, et al. 2016. J Immunol. 197: 1788 - 1800. PubMed Deng P, et al. 2021. Cell Stem Cell. 28(6):1057-1073.e7. PubMed Bao Z, et al. 2020. Ann Transplant. 25:e921287. PubMed Bajaña S, et al. 2022. iScience. 25:103732. PubMed Koide S, et al. 2022. iScience. 25:103603. PubMed Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed Wilkinson AC, et al. 2019. Nature. 571:117. PubMed Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed\u003cbr\u003e\nRRID: AB_313342 (BioLegend Cat. No. 108105) AB_313343 (BioLegend Cat. No. 108106)\u003cbr\u003e\nStructure: Ly-6 multigene family, 18 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells\u003cbr\u003e\nFunction: Regulates B and T cell responses\u003cbr\u003e\nCell Type: B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nAntigen References: 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709.\u003cbr\u003e\nGene ID: 110454\u003cbr\u003e\nUniProt: View information about Ly-6A\/E on UniProt.org\u003cbr\u003e\nClone: D7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Sca-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863002239145,"sku":"108105","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-108105","provider":"Iright","version":"1.0","type":"link"}