{"product_id":"biolegend-108306","title":"Biolegend, 108306, FITC anti-mouse Ly-51 Antibody, 500μg","description":"\u003cp\u003eLy-51 is a 140 kD protein also known as 6C3\/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: C57BL\/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP) Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP) Adkins B, et al. 1988. Immunogenetics 27:180. (IHC) Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC) Goverman J, et al. 1997. Immunol. Today 18:204. (IHC) Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC) Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)\u003cbr\u003e\nProduct Citations: Weng L, et al. 2016. Sci Rep. 6:38632. PubMed Sitte S, et al. 2012. J Immunol. 188:2677. PubMed Chou W, et al. 2006. Blood. 108:3005. PubMed Lin C, et al. 2022. Nat Commun. 13:6869. PubMed Xiao G, et al. 2018. Cell. 173:470. PubMed Miragaia RJ, et al. 2018. Sci Rep. 8:685. PubMed Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed Agarwal P, et al. 2021. Cell Reports. 36(2):109386. PubMed Su M, et al. 2018. Immunol Cell Biol. 9:2448. PubMed Wang L, et al. 2020. Nat Commun. 0.654166667. PubMed Han Y, et al. 2019. J Clin Invest. 130:26. PubMed\u003cbr\u003e\nRRID: AB_313362 (BioLegend Cat. No. 108305)\u003cbr\u003e\nStructure: Aminopeptidase A, 140 kD\u003cbr\u003e\nDistribution: B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells\u003cbr\u003e\nFunction: Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Epithelial cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nAntigen References: 1. Goverman J, et al. 1997. Immunol. Today 18:204. 2. Morse HC, et al. 1987. J. Exp. Med. 165:920. 3. Sherwood P, et al. 1990. Int. Immunol. 2:399. 4. Lin Q, et al. 1998. J. Immunol. 160:4681.\u003cbr\u003e\nGene ID: 13809\u003cbr\u003e\nUniProt: View information about Ly-51 on UniProt.org\u003cbr\u003e\nClone: 6C3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: 6C3\/BP-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862474182825,"sku":"108306","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-108306","provider":"Iright","version":"1.0","type":"link"}