{"product_id":"biolegend-109807","title":"Biolegend, 109807, PE anti-mouse CD45.2 Antibody, 50μg","description":"\u003cp\u003eCD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB\/c, CBA\/Ca, CBA\/J, C3H\/He, C57BL, C57BR, C57L, C58, DBA\/1, DBA\/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: B10.S mouse thymocytes and splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Suzuki K, et al. 2000. Immunity 13:691. (IHC) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837. Pascal V, et al. 2007. J. Immunol. 179:1751. Burman AC, et al. 2007. Blood 110:1064. Kincaid EZ, et al. 2007. J. Immunol. 179:3187. Phan TG, et al. 2007. Nature Immunol. 8:992. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed Wen T, et al. 2013. PNAS. 110:6067. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed\u003cbr\u003e\nProduct Citations: Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed Melzi E, et al. 2022. Immunity. 55:2168. PubMed Yi C, et al. 2023. J Neuroinflammation. 20:45. PubMed Moriyama M, et al. 2023. Proc Natl Acad Sci U S A. 120:e2221652120. PubMed Oh J, et al. 2023. Nat Commun. 14:3278. PubMed Takihara Y, et al. 2022. Commun Biol. 5:776. PubMed Lehmkuhl P, et al. 2021. Cell Mol Immunol. 18:1677. PubMed Ozga AJ, et al. 2022. Immunity. 55:82. PubMed He D, et al. 2022. Immunity. 55:159. PubMed Wang H, et al. 2022. Immunol Cell Biol. 100:547. PubMed Nozaki K, et al. 2022. Nature. 606:960. PubMed Shemesh A, et al. 2022. J Exp Med. 219: . PubMed Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed Nishina T, et al. 2023. iScience. 26:105934. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed De Giovanni M, et al. 2022. Cell. 185:815. PubMed Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed Lee S, et al. 2021. JCI Insight. 6:. PubMed Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed Yang X, et al. 2020. Blood Sci. 2:89. PubMed Bonnet C, et al. 2021. iScience. 24:103399. PubMed Davila S, et al. 2014. J Immunol. 192:4284. PubMed Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed Yang F, et al. 2020. Cancer Res. 80:3677. PubMed Sugimoto C, et al. 2022. Elife. 11:. PubMed Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed Amor C, et al. 2020. Nature. 583:127. PubMed Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed Rengarajan S, et al. 2020. Cell Rep Med. :1. PubMed He L, et al. 2019. iScience. 0.790972222. PubMed Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Kinkel SA, et al. 2022. iScience. 25:104684. PubMed He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed Chen D, et al. 2021. Molecular Cell. 81:3833. PubMed Kim C, et al. 2019. Cell Rep. 29:2202. PubMed Russler-Germain EV, et al. 2021. Elife. 10:. PubMed Mansell E, et al. 2020. Cell Stem Cell. . PubMed Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed Markey K, et al. 2014. J Immunol. 192:5426. PubMed Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed Ndeupen S, et al. 2022. STAR Protoc. 3:101350. PubMed Zhang X, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed Tang Q, et al. 2021. Immunity. 54:1961. PubMed Wang X, et al. 2021. EMBO J. 40:e105926. PubMed Hirata T, et al. 2012. Int Immunol. 24:705. PubMed Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed Fang F, et al. 2021. Cell Rep. 37:109981. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Xiong J, et al. 2011. Int Immunol. 23:56. PubMed Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed Chen C, et al. 2020. Cell Rep. 2136:30. PubMed Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed Wang X, et al. 2021. Cell. 184:5357. PubMed Koide S, et al. 2022. iScience. 25:103603. PubMed Sun Y, et al. 2020. J Immunol. 205:2649. PubMed Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed Sindrilaru A, et al. 2009. Blood. 113:5266. PubMed Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed Wang X, et al. 2022. Elife. 11:. PubMed Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed Huang X, et al. 2021. Immunity. . PubMed Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed Spath S, et al. 2022. iScience. 25:104998. PubMed Young K, et al. 2021. Cell Stem Cell. . PubMed Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed Finkin S et al. 2019. Immunity. 51(2):324-336 . PubMed Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed\u003cbr\u003e\nRRID: AB_313444 (BioLegend Cat. No. 109807) AB_313445 (BioLegend Cat. No. 109808)\u003cbr\u003e\nStructure: Protein tyrosine phosphatase (PTP) family, 180-240 kD\u003cbr\u003e\nDistribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice\u003cbr\u003e\nFunction: Phosphatase, T and B cell activation\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, CD4\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.\u003cbr\u003e\nGene ID: 19264\u003cbr\u003e\nUniProt: View information about CD45.2 on UniProt.org\u003cbr\u003e\nClone: 104\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ly-5.2, LCA\u003cbr\u003e\nIsotype: Mouse (SJL) IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860771524777,"sku":"109807","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-109807","provider":"Iright","version":"1.0","type":"link"}