{"product_id":"biolegend-109901","title":"Biolegend, 109901, Purified anti-mouse I-A, 50μg","description":"\u003cp\u003eThe 10-3.6 antibody reacts with the β chain of the I-A k MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 k bearing mice and involved in antigen presentation to T cells expressing CD3\/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-A f,r,s antigens and I-A g7 of NOD mice; it does not react with other haplotypes ( e.g., b, d, p, q).\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C3H mouse splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include immunoprecipitation1,2, protection against autoimmune IDDM3, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth4, and immunohistochemical staining5 of acetone-fixed frozen sections. This clone does not cross react with other haplotypes (e.g. b, d, p, q).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Landais D, et al. 1986. J. Immunol. 137:3002. (IP) Kupinski JM, et al. 1983. J. Immunol. 130:2277. (IP) Kappler JW, et al. 1981. J. Exp. Med. 153:1198. Alisauskas RM, et al. 1986. Immunopharmacology 12:1. Reis e Sousa and Germain 1999. J. Immunol. 162:6652. (IHC) Yui MA, et al. 2010. J. Immunol. 185:284. PubMed Gaudreau S, et al. 2007. J. Immunol. 179:3638. (FC)\u003cbr\u003e\nProduct Citations: Drake JR, et al. 2019. Immunohorizons. 3:28. PubMed Wu X, et al. 2022. J Neuroinflammation. 19:253. PubMed Khosravi-Maharlooei M, et al. 2021. J Autoimmun. 119:102612. PubMed\u003cbr\u003e\nRRID: AB_313451 (BioLegend Cat. No. 109902)\u003cbr\u003e\nStructure: β chain of MHC class II\u003cbr\u003e\nDistribution: B cell and activated T cells, APCs of H-2b mice\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD4\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.\u003cbr\u003e\nGene ID: 14961\u003cbr\u003e\nUniProt: View information about I-Ak on UniProt.org\u003cbr\u003e\nClone: 10-3.6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class II\u003cbr\u003e\nIsotype: Mouse (CWB) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860767101097,"sku":"109901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-109901","provider":"Iright","version":"1.0","type":"link"}