{"product_id":"biolegend-110001","title":"Biolegend, 110001, Purified anti-mouse I-A, 50μg","description":"\u003cp\u003eThe 11-5.2 antibody reacts with the α chain of the I-A k MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 k bearing mice and are involved in antigen presentation to T cells expressing CD3\/TCR and CD4 proteins. The 11-5.2 antibody cross-reacts with cells from mice bearing the H-2 r haplotype. Reactivity with other H-2 haplotypes ( e.g., b, d, f, p, q, s) has not been observed. The 11-5.2 antibody has been reported to block antigen presentation and induce B cell differentiation.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CKB mouse splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of MHC class II - restricted CD4+ T cell responses2,3, induction of B cell differentiation4, and immunohistochemical staining5 of acetone-fixed frozen sections. This clone does not cross react with other haplotypes (e.g., b, d, f, p, q, s).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Oi VT, et al. 1978. Curr. Top. Microbiol. Immunol. 81:115. (IP) Formby B, et al. 1990. P. Natl. Acad. Sci. USA 87:2438. Kappler JW, et al. 1981. J. Exp. Med. 153:1198. Bishop GA, et al. 1989. P. Natl. Acad. Sci. USA 86:5933. Ardehali A, et al. 1995. Circulation 92:450. (IHC) Turnquist HR, et al. 2007. J. Immunol. 178:7018. Zhang L, et al. 2011. Lupus. 20:667. PubMed.\u003cbr\u003e\nProduct Citations: Zhang L, et al. 2011. Lupus. 1.296527778. PubMed Hausmann A, et al. 2021. J Exp Med. 218:. PubMed Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed\u003cbr\u003e\nRRID: AB_313459 (BioLegend Cat. No. 110002)\u003cbr\u003e\nStructure: α chain of MHC class II\u003cbr\u003e\nDistribution: B cell and activated T cells, APCs of H-2b mice\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD4\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.\u003cbr\u003e\nGene ID: 14960\u003cbr\u003e\nUniProt: View information about I-Ak on UniProt.org\u003cbr\u003e\nClone: 11-5.2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class II\u003cbr\u003e\nIsotype: Mouse (BALB\/c) IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860786204841,"sku":"110001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-110001","provider":"Iright","version":"1.0","type":"link"}