{"product_id":"biolegend-110205","title":"Biolegend, 110205, FITC anti-mouse I-E, 50μg","description":"\u003cp\u003eThe 14-4-4S antibody reacts with the I-E k MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 k bearing mice and are involved in antigen presentation to T cells expressing CD3\/TCR and CD4 proteins. The 14-4-4S antibody cross-reacts with cells from mice of the H-2 d , H-2 p , and H-2 r haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2 b , H-2 f , H-2 q , and H-2 s haplotypes do not express I-E antigen. The 14-4-4S antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-E k .\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C3H mouse skin graft and splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2, in vitro induction of B-cell differentiation3, in vitro blocking of antigen presentation4, and immunohistochemical staining5,6 of acetone-fixed frozen sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ozato K, et al. 1980. J. Immunol. 124:533. Nei U, et al. 1994. Int. Immunol. 6:61. (IP) Harton JA, et al. 1991. Immunogenetics 34:358. Roy M, et al. 1995. Eur. J. Immunol. 25:596. Farr A, et al. 1996. Eur. J. Immunol. 26:3185. (IHC) Desmet C, et al. 2004. J. Immunol. 173:5766. (IHC) Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)\u003cbr\u003e\nRRID: AB_313470 (BioLegend Cat. No. 110205)\u003cbr\u003e\nStructure: MHC class II\u003cbr\u003e\nDistribution: B cell and activated T cells, APCs of H-2b mice\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD4\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.\u003cbr\u003e\nGene ID: 14969\u003cbr\u003e\nUniProt: View information about I-Ek on UniProt.org\u003cbr\u003e\nClone: 14-4-4S\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: I-Ek, MHC class II\u003cbr\u003e\nIsotype: Mouse (C3H.SW) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860801900713,"sku":"110205","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-110205","provider":"Iright","version":"1.0","type":"link"}