{"product_id":"biolegend-110715","title":"Biolegend, 110715, APC\/Cyanine7 anti-mouse CD45.1 Antibody, 25μg","description":"\u003cp\u003eCD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL\/J, STS\/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: SJL mouse thymocytes and splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed\u003cbr\u003e\nProduct Citations: Jash A, et al. 2016. J Immunol. 197: 1159 - 1168. PubMed Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed Wang D, et al. 2022. Cell Rep. 39:110587. PubMed VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed Zhao M, et al. 2022. Nat Commun. 13:7600. PubMed Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed North K, et al. 2022. Nat Biotechnol. 40:1103. PubMed Morris V, et al. 2022. Cell Rep. 39:110752. PubMed Pulous FE, et al. 2022. Nat Neurosci. 25:567. PubMed Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed Le Q, et al. 2021. Clin Cancer Res. Online ahead of prin. PubMed Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed Frame JM, et al. 2020. Developmental Cell. 55(2):133-149.e6. PubMed Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed Amor C, et al. 2020. Nature. 583:127. PubMed Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed Benechet AP, et al. 2019. Nature. 574:200. PubMed Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed Fu H, et al. 2020. Front Immunol. 11:595813. PubMed Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed Li J, et al. 2018. Immunity. 49:178. PubMed Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed Sun L, et al. 2021. Cell Reports. 35(9):109188. PubMed Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed De Simone G, et al. 2021. Immunity. :. PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed Burgess M, et al. 2014. Blood. 124:3947. PubMed Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed Chen F, et al. 2022. Cell Rep. 38:110215. PubMed Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Wang X, et al. 2022. Elife. 11:. PubMed Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed Chen S, et al. 2018. Nat Commun. 9:5298. PubMed Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed\u003cbr\u003e\nRRID: AB_313504 (BioLegend Cat. No. 110715) AB_313505 (BioLegend Cat. No. 110716)\u003cbr\u003e\nStructure: Protein tyrosine phosphatase (PTP) family, 180-240 kD\u003cbr\u003e\nDistribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice\u003cbr\u003e\nFunction: Phosphatase, T and B cell activation\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, CD4\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.\u003cbr\u003e\nGene ID: 19264\u003cbr\u003e\nUniProt: View information about CD45.1 on UniProt.org\u003cbr\u003e\nClone: A20\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T200, Ly-5.1, LCA\u003cbr\u003e\nIsotype: Mouse (A.SW) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862519795881,"sku":"110715","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-110715","provider":"Iright","version":"1.0","type":"link"}